BROTHER of FT and TFL1 (BFT) has TFL1-like activity and functions redundantly with TFL1 in inflorescence meristem development in Arabidopsis

Seong Jeon Yoo, Kyung Sook Chung, Seung Hye Jung, So Yeon Yoo, Jong Seob Lee, Ji Hoon Ahn

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The FLOWERING LOCUS T (FT)/TERMINAL FLOWER 1 (TFL1) family is a small gene family that encodes important regulators that control flower development in Arabidopsis. Here, we investigated the biological role of the product of BROTHER OF FT AND TFL1 (BFT), a member of this family, whose function remains unknown. Comparison of the critical residues that play a role in distinguishing FT- or TFL1-like activity revealed that BFT is more similar to FT. Similar to FT expression, BFT expression showed a diurnal oscillation pattern, peaking in the evening. In situ hybridization revealed BFT expression in the shoot apical meristem, young leaf and axillary inflorescence meristem. Transgenic plants over-expressing BFT exhibited delayed flowering and severe floral defects (floral indeterminacy and compact inflorescences surrounded by serrate leaves), similar to 35S::TFL1 plants. LEAFY (LFY) and APETALA1 (AP1) expression was significantly reduced in 35S::BFT plants. BFT over-expression failed to rescue the terminal flower phenotype of tfl1 mutants; however, it delayed both terminal flower formation in the primary inflorescence and axillary inflorescence development in the tfl1 mutant background. Consistent with this, the loss-of-function BFT alleles, bft-2 and an BFT RNAi line, accelerated termination of the primary inflorescence and formation of axillary inflorescences in the tfl1 mutant background. Taken together, our results suggest that, despite similarities in the critical residues of BFT and FT, BFT possesses a TFL1-like activity and functions redundantly with TFL1 in inflorescence meristem development, and possibly contributes to the regulation of plant architecture.

Original languageEnglish
Pages (from-to)241-253
Number of pages13
JournalPlant Journal
Volume63
Issue number2
DOIs
Publication statusPublished - 2010 Jul 1

Fingerprint

Inflorescence
Meristem
Arabidopsis
meristems
inflorescences
flowering
flowers
loci
mutants
Genetically Modified Plants
shoot meristems
plant architecture
apical meristems
RNA Interference
in situ hybridization
In Situ Hybridization
oscillation
leaves
transgenic plants
Alleles

Keywords

  • Arabidopsis
  • BROTHER OF FT AND TFL1
  • FLOWERING LOCUS T
  • flowering time
  • inflorescence meristem
  • TERMINAL FLOWER 1

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

BROTHER of FT and TFL1 (BFT) has TFL1-like activity and functions redundantly with TFL1 in inflorescence meristem development in Arabidopsis. / Yoo, Seong Jeon; Chung, Kyung Sook; Jung, Seung Hye; Yoo, So Yeon; Lee, Jong Seob; Ahn, Ji Hoon.

In: Plant Journal, Vol. 63, No. 2, 01.07.2010, p. 241-253.

Research output: Contribution to journalArticle

Yoo, Seong Jeon ; Chung, Kyung Sook ; Jung, Seung Hye ; Yoo, So Yeon ; Lee, Jong Seob ; Ahn, Ji Hoon. / BROTHER of FT and TFL1 (BFT) has TFL1-like activity and functions redundantly with TFL1 in inflorescence meristem development in Arabidopsis. In: Plant Journal. 2010 ; Vol. 63, No. 2. pp. 241-253.
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