Bryostatin-1 promotes long-term potentiation via activation of PKCα and PKCε in the hippocampus

H. Kim, S. H. Han, H. Y. Quan, Y. J. Jung, J. An, P. Kang, J. B. Park, B. J. Yoon, G. H. Seol, S. S. Min

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Activation of protein kinase C (PKC) by bryostatin-1 affects various functions of the central nervous system. We explored whether bryostatin-1 influenced synaptic plasticity via a process involving PKC. Our purpose was to examine whether bryostatin-1 affected the induction of hippocampal long-term potentiation (LTP) in Schaffer-collateral fibers (CA1 fibers) of the hippocampus, and/or influenced the intracellular Ca2+ level of hippocampal neurons. We also determined the PKC isoforms involved in these processes. We found that bryostatin-1 strongly facilitated LTP induction, in a dose-dependent manner, upon single-theta burst stimulation (TBS). Further, intracellular Ca2+ levels also increased with increasing concentration of bryostatin-1. The facilitative effects of bryostatin-1 in terms of LTP induction and enhancement of intracellular Ca2+ levels were blocked by specific inhibitors of PKCα and PKCε, but not of PKCδ. Our results suggest that bryostatin-1 is involved in neuronal functioning and facilitates induction of LTP via activation of PKCα and/or PKCε.

Original languageEnglish
Pages (from-to)348-355
Number of pages8
JournalNeuroscience
Volume226
DOIs
Publication statusPublished - 2012 Dec 13

Keywords

  • Bryostatin-1
  • Hippocampus
  • Long-term potentiation (LTP)
  • Protein kinase C (PKC)
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Bryostatin-1 promotes long-term potentiation via activation of PKCα and PKCε in the hippocampus'. Together they form a unique fingerprint.

Cite this