Bryostatin-1 promotes long-term potentiation via activation of PKCα and PKCε in the hippocampus

H. Kim, S. H. Han, H. Y. Quan, Y. J. Jung, J. An, P. Kang, J. B. Park, Bongjune Yoon, Geun Hee Seol, S. S. Min

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Activation of protein kinase C (PKC) by bryostatin-1 affects various functions of the central nervous system. We explored whether bryostatin-1 influenced synaptic plasticity via a process involving PKC. Our purpose was to examine whether bryostatin-1 affected the induction of hippocampal long-term potentiation (LTP) in Schaffer-collateral fibers (CA1 fibers) of the hippocampus, and/or influenced the intracellular Ca2+ level of hippocampal neurons. We also determined the PKC isoforms involved in these processes. We found that bryostatin-1 strongly facilitated LTP induction, in a dose-dependent manner, upon single-theta burst stimulation (TBS). Further, intracellular Ca2+ levels also increased with increasing concentration of bryostatin-1. The facilitative effects of bryostatin-1 in terms of LTP induction and enhancement of intracellular Ca2+ levels were blocked by specific inhibitors of PKCα and PKCε, but not of PKCδ. Our results suggest that bryostatin-1 is involved in neuronal functioning and facilitates induction of LTP via activation of PKCα and/or PKCε.

Original languageEnglish
Pages (from-to)348-355
Number of pages8
JournalNeuroscience
Volume226
DOIs
Publication statusPublished - 2012 Dec 13

Fingerprint

Long-Term Potentiation
Protein Kinase C
Hippocampus
Neuronal Plasticity
bryostatin 1
protein kinase C kinase
Protein Isoforms
Central Nervous System
Neurons

Keywords

  • Bryostatin-1
  • Hippocampus
  • Long-term potentiation (LTP)
  • Protein kinase C (PKC)
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kim, H., Han, S. H., Quan, H. Y., Jung, Y. J., An, J., Kang, P., ... Min, S. S. (2012). Bryostatin-1 promotes long-term potentiation via activation of PKCα and PKCε in the hippocampus. Neuroscience, 226, 348-355. https://doi.org/10.1016/j.neuroscience.2012.08.055

Bryostatin-1 promotes long-term potentiation via activation of PKCα and PKCε in the hippocampus. / Kim, H.; Han, S. H.; Quan, H. Y.; Jung, Y. J.; An, J.; Kang, P.; Park, J. B.; Yoon, Bongjune; Seol, Geun Hee; Min, S. S.

In: Neuroscience, Vol. 226, 13.12.2012, p. 348-355.

Research output: Contribution to journalArticle

Kim, H. ; Han, S. H. ; Quan, H. Y. ; Jung, Y. J. ; An, J. ; Kang, P. ; Park, J. B. ; Yoon, Bongjune ; Seol, Geun Hee ; Min, S. S. / Bryostatin-1 promotes long-term potentiation via activation of PKCα and PKCε in the hippocampus. In: Neuroscience. 2012 ; Vol. 226. pp. 348-355.
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