BSA as additive

A simple strategy for practical applications of PNA in bioanalysis

Jieon Lee, Il Soo Park, Henna Kim, Jae Sung Woo, Byong Seok Choi, Dal Hee Min

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Application of peptide nucleic acid (PNA) in bioanalysis has been limited due to its nonspecific adsorption onto hydrophobic surface in spite of favorable properties such as higher chemical/biological stability, specificity and binding affinity towards target nucleic acids compared to natural nucleic acid probes. Herein, we employed BSA in PNA application to enhance the stability of PNA in hydrophobic containers and improve the sensing performance of the DNA sensor based on graphene oxide (GO) and PNA. Addition of 0.01% BSA in a PNA solution effectively prevented the adsorption of PNA on hydrophobic surface and increased the portion of the effective PNA strands for target binding without interfering duplex formation with a complementary target sequence. In the GO based biosensor using PNA, BSA interrupted the unfavorable adsorption of PNA/DNA duplex on GO surface, while allowing the adsorption of ssPNA, resulting in improvement of the performance of the DNA sensor system by reducing the detection limit by 90-folds.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalBiosensors and Bioelectronics
Volume69
DOIs
Publication statusPublished - 2015 Jul 5
Externally publishedYes

Fingerprint

Peptide Nucleic Acids
Adsorption
Graphite
Oxides
Graphene
DNA
Nucleic acids
Nucleic Acid Probes
Sensors
Biosensing Techniques
Biosensors
Nucleic Acids
Containers
Limit of Detection

Keywords

  • Bioanalysis
  • Bovine serum albumin
  • Graphene oxide
  • Peptide nucleic acid
  • Signal enhancement

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

BSA as additive : A simple strategy for practical applications of PNA in bioanalysis. / Lee, Jieon; Park, Il Soo; Kim, Henna; Woo, Jae Sung; Choi, Byong Seok; Min, Dal Hee.

In: Biosensors and Bioelectronics, Vol. 69, 05.07.2015, p. 167-173.

Research output: Contribution to journalArticle

Lee, Jieon ; Park, Il Soo ; Kim, Henna ; Woo, Jae Sung ; Choi, Byong Seok ; Min, Dal Hee. / BSA as additive : A simple strategy for practical applications of PNA in bioanalysis. In: Biosensors and Bioelectronics. 2015 ; Vol. 69. pp. 167-173.
@article{8646156688a7470386e6fd7370bfb582,
title = "BSA as additive: A simple strategy for practical applications of PNA in bioanalysis",
abstract = "Application of peptide nucleic acid (PNA) in bioanalysis has been limited due to its nonspecific adsorption onto hydrophobic surface in spite of favorable properties such as higher chemical/biological stability, specificity and binding affinity towards target nucleic acids compared to natural nucleic acid probes. Herein, we employed BSA in PNA application to enhance the stability of PNA in hydrophobic containers and improve the sensing performance of the DNA sensor based on graphene oxide (GO) and PNA. Addition of 0.01{\%} BSA in a PNA solution effectively prevented the adsorption of PNA on hydrophobic surface and increased the portion of the effective PNA strands for target binding without interfering duplex formation with a complementary target sequence. In the GO based biosensor using PNA, BSA interrupted the unfavorable adsorption of PNA/DNA duplex on GO surface, while allowing the adsorption of ssPNA, resulting in improvement of the performance of the DNA sensor system by reducing the detection limit by 90-folds.",
keywords = "Bioanalysis, Bovine serum albumin, Graphene oxide, Peptide nucleic acid, Signal enhancement",
author = "Jieon Lee and Park, {Il Soo} and Henna Kim and Woo, {Jae Sung} and Choi, {Byong Seok} and Min, {Dal Hee}",
year = "2015",
month = "7",
day = "5",
doi = "10.1016/j.bios.2015.02.030",
language = "English",
volume = "69",
pages = "167--173",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - BSA as additive

T2 - A simple strategy for practical applications of PNA in bioanalysis

AU - Lee, Jieon

AU - Park, Il Soo

AU - Kim, Henna

AU - Woo, Jae Sung

AU - Choi, Byong Seok

AU - Min, Dal Hee

PY - 2015/7/5

Y1 - 2015/7/5

N2 - Application of peptide nucleic acid (PNA) in bioanalysis has been limited due to its nonspecific adsorption onto hydrophobic surface in spite of favorable properties such as higher chemical/biological stability, specificity and binding affinity towards target nucleic acids compared to natural nucleic acid probes. Herein, we employed BSA in PNA application to enhance the stability of PNA in hydrophobic containers and improve the sensing performance of the DNA sensor based on graphene oxide (GO) and PNA. Addition of 0.01% BSA in a PNA solution effectively prevented the adsorption of PNA on hydrophobic surface and increased the portion of the effective PNA strands for target binding without interfering duplex formation with a complementary target sequence. In the GO based biosensor using PNA, BSA interrupted the unfavorable adsorption of PNA/DNA duplex on GO surface, while allowing the adsorption of ssPNA, resulting in improvement of the performance of the DNA sensor system by reducing the detection limit by 90-folds.

AB - Application of peptide nucleic acid (PNA) in bioanalysis has been limited due to its nonspecific adsorption onto hydrophobic surface in spite of favorable properties such as higher chemical/biological stability, specificity and binding affinity towards target nucleic acids compared to natural nucleic acid probes. Herein, we employed BSA in PNA application to enhance the stability of PNA in hydrophobic containers and improve the sensing performance of the DNA sensor based on graphene oxide (GO) and PNA. Addition of 0.01% BSA in a PNA solution effectively prevented the adsorption of PNA on hydrophobic surface and increased the portion of the effective PNA strands for target binding without interfering duplex formation with a complementary target sequence. In the GO based biosensor using PNA, BSA interrupted the unfavorable adsorption of PNA/DNA duplex on GO surface, while allowing the adsorption of ssPNA, resulting in improvement of the performance of the DNA sensor system by reducing the detection limit by 90-folds.

KW - Bioanalysis

KW - Bovine serum albumin

KW - Graphene oxide

KW - Peptide nucleic acid

KW - Signal enhancement

UR - http://www.scopus.com/inward/record.url?scp=84923637597&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923637597&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2015.02.030

DO - 10.1016/j.bios.2015.02.030

M3 - Article

VL - 69

SP - 167

EP - 173

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

ER -