Burkholderia megalochromosomata sp. nov., isolated from grassland soil

Inwoo Baek, Boram Seo, Imchang Lee, Kihyun Lee, Sang Cheol Park, Hana Yi, Jongsik Chun

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A Gram-stain negative, rod-shaped, non-spore-forming, obligate aerobic bacterial strain, JC2949T, was isolated from grassland soil in Gwanak Mountain, Seoul, Republic of Korea. Phylogenetic analysis, based on 16S rRNA sequences, indicated that strain JC2949T belongs to the genus Burkholderia, showing highest sequence similarities with Burkholderia grimmiae R27T (98.8%), Burkholderia cordobensis LMG 27620T (98.6%), Burkholderia jiangsuensis MP-1TT(98.6%), Burkholderia zhejiangensis OP-1T (98.5%), Burkholderia humi LMG 22934T (97.5%), Burkholderia terrestris LMG 22937T (97.3%), Burkholderia telluris LMG 22936T (97.2%) and Burkholderia glathei ATCC 29195T (97.0%). The major fatty acids of strain JC2949T were C18: 1ω7c, summed feature 3 (C16: 1ω7c and/or C16: 1ω6c) and C16: 0. Its predominant polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unknown amino phospholipid. The dominant isoprenoid quinone was ubiquinone Q-8. The pairwise average nucleotide identity values between strain JC2949T and the genomes of 30 other species of the genus Burkholderia ranged from 73.4-90.4%, indicating that the isolate is a novel genomic species within this genus. Based on phenotypic and chemotaxonomic comparisons, it is clear that strain JC2949T represents a novel species of the genus Burkholderia. We propose the name for this novel species to be Burkholderia megalochromosomata sp. nov. The type strain is JC2949T (=KACC 17925T=JCM 19905T

Original languageEnglish
Pages (from-to)959-964
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number3
DOIs
Publication statusPublished - 2015

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Burkholderia
grassland soil
grassland soils
Soil
Soils
new species
isoprenoid
phospholipid
genomics
Phosphatidylglycerols
Cardiolipins
Ubiquinone
fatty acid
genome
lipid
Terpenes
Burkholderia glathei
phylogenetics
mountain
Grassland

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Burkholderia megalochromosomata sp. nov., isolated from grassland soil. / Baek, Inwoo; Seo, Boram; Lee, Imchang; Lee, Kihyun; Park, Sang Cheol; Yi, Hana; Chun, Jongsik.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 3, 2015, p. 959-964.

Research output: Contribution to journalArticle

Baek, Inwoo ; Seo, Boram ; Lee, Imchang ; Lee, Kihyun ; Park, Sang Cheol ; Yi, Hana ; Chun, Jongsik. / Burkholderia megalochromosomata sp. nov., isolated from grassland soil. In: International Journal of Systematic and Evolutionary Microbiology. 2015 ; Vol. 65, No. 3. pp. 959-964.
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abstract = "A Gram-stain negative, rod-shaped, non-spore-forming, obligate aerobic bacterial strain, JC2949T, was isolated from grassland soil in Gwanak Mountain, Seoul, Republic of Korea. Phylogenetic analysis, based on 16S rRNA sequences, indicated that strain JC2949T belongs to the genus Burkholderia, showing highest sequence similarities with Burkholderia grimmiae R27T (98.8{\%}), Burkholderia cordobensis LMG 27620T (98.6{\%}), Burkholderia jiangsuensis MP-1TT(98.6{\%}), Burkholderia zhejiangensis OP-1T (98.5{\%}), Burkholderia humi LMG 22934T (97.5{\%}), Burkholderia terrestris LMG 22937T (97.3{\%}), Burkholderia telluris LMG 22936T (97.2{\%}) and Burkholderia glathei ATCC 29195T (97.0{\%}). The major fatty acids of strain JC2949T were C18: 1ω7c, summed feature 3 (C16: 1ω7c and/or C16: 1ω6c) and C16: 0. Its predominant polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unknown amino phospholipid. The dominant isoprenoid quinone was ubiquinone Q-8. The pairwise average nucleotide identity values between strain JC2949T and the genomes of 30 other species of the genus Burkholderia ranged from 73.4-90.4{\%}, indicating that the isolate is a novel genomic species within this genus. Based on phenotypic and chemotaxonomic comparisons, it is clear that strain JC2949T represents a novel species of the genus Burkholderia. We propose the name for this novel species to be Burkholderia megalochromosomata sp. nov. The type strain is JC2949T (=KACC 17925T=JCM 19905T",
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