Burkholderia monticola sp. Nov., isolated from mountain soil

An ivory/yellow, Gram-stain-negative, short-rod-shaped, aerobic bacterial strain, designated JC2948^T, was isolated from a soil sample taken from Gwanak Mountain, Republic of Korea. 16S rRNA gene sequence analysis indicated that strain JC2948^T belongs to the genus Burkholderia. The test strain showed highest sequence similarities to Burkholderia tropica LMG 22274^T(97.6%), Burkholderia acidipaludis NBRC 101816^T (97.5%), Burkholderia tuberum LMG 21444^T (97.5%), Burkholderia sprentiae LMG 27175^T (97.4%), Burkholderia terricola LMG 20594^T (97.3%) and Burkholderia diazotrophica LMG 26031^T (97.1%). Based on average nucleotide identity (ANI) values, the new isolate represents a novel genomic species as it shows less than 90% ANI values with other closely related species. Also, other phylosiological and biochemical comparisons allowed the phenotypic differentiation of strain JC2948^T from other members of the genus Burkholderia. Therefore, we suggest that this strain should be classified as the type strain of a novel species of the genus Burkholderia. The name Burkholderia monticola sp. nov. (type strain, JC2948^T5JCM 19904^T5KACC 17924^T) is proposed.