Butyrate-induced differentiation of PC12 cells to chromaffin cells involves cell adhesion and induction of extracellular proteins and cell adhesion proteins

Jee In Heoa, Soo Jin Oh, Yoon Jung Kho, Jeong hyeon Kim, Hong Joon Kang, Seong Hoon Park, Hyun Seok Kim, Jong Yeon Shin, Sung Young Lee, Min Ju Kim, Bon Hong Min, Sung Chan Kim, Jae Bong Park, Jaebong Kim, Jae Yong Lee

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3 Citations (Scopus)

Abstract

PC12 cells were differentiated into the cells of chromaffin phenotype by butyrate treatment. Cells were aggregated and formed tight cell adhesion. To investigate the molecular change in this differentiation, we examined expression levels of cell adhesion proteins and extracellular proteins during butyrate induced-differentiation of PC12 cells. Integrin β1, integrin α7, E cadherin, VCAM, collagen-I, fibronectin, desmoglein and connexin were increased during differentiation. The levels of clusterin and secreted clusterin were also increased. These increased levels of cell adhesion proteins and extracellular proteins appear to induce cell aggregation and tight cell adhesion. The levels of p21, p27 and p16 were increased probably because of differentiation-related growth arrest during differentiation. Prolonged incubation of butyrate up to 1 day was required for differentiation. Signal transduction pathways for this differentiatiom could not be identified since various inhibitors had no effect. The results showed that butyrateinduced differentiation of PC12 cells to chromaffin cells involves tight cell adhesion and induction of extracellular proteins and cell adhesion proteins.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalAnimal Cells and Systems
Volume14
Issue number4
DOIs
Publication statusPublished - 2010

Keywords

  • Butyrate
  • Cell adhesion proteins
  • Chromaffin cells
  • Differentiation
  • Pc12

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Heoa, J. I., Oh, S. J., Kho, Y. J., Kim, J. H., Kang, H. J., Park, S. H., Kim, H. S., Shin, J. Y., Lee, S. Y., Kim, M. J., Min, B. H., Kim, S. C., Park, J. B., Kim, J., & Lee, J. Y. (2010). Butyrate-induced differentiation of PC12 cells to chromaffin cells involves cell adhesion and induction of extracellular proteins and cell adhesion proteins. Animal Cells and Systems, 14(4), 261-266. https://doi.org/10.1080/19768354.2010.528204