C-C motif chemokine ligand 23 abolishes ER stress- and LPS-induced reduction in proliferation of bovine endometrial epithelial cells

Whasun Lim, Hyocheol Bae, Fuller W. Bazer, Gwonhwa Song

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

To reduce embryonic losses in domestic animals for economic production of livestock meat and milk, chemokines and their receptors are required for proper implantation and placentation during early pregnancy. In this study, we investigated the effects of C-C-motif chemokine ligand 23 (CCL23) on the proliferation of bovine endometrial (BEND) epithelial cells. CCL23 treatment improved BEND cell proliferation by enhancing PCNA and cyclin D1 expression via activation of the PI3K/AKT and MAPK signaling pathways. In addition, a combination of CCL23 and tunicamycin reversed the ER stress-induced reduction in cell proliferation and the decreased expression of UPR-mediated signaling proteins, including IRE1α, PERK, and ATF6α. Moreover, it regulated the lipopolysaccharide-induced inflammation in BEND cells by inhibiting the expression of pro-inflammatory cytokines (IL-6 and IL-8), and by restoring intracellular Ca2+ levels. These findings demonstrate that CCL23 improves endometrial development and uterine receptivity required for implantation and placentation during early pregnancy.

Original languageEnglish
Pages (from-to)3529-3539
Number of pages11
JournalJournal of Cellular Physiology
Volume233
Issue number4
DOIs
Publication statusPublished - 2018 Apr 1

Keywords

  • CCL23
  • endometrial epithelial cell
  • proliferation
  • signal transduction

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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