C-Fos regulates hepatitis C virus propagation

Sang Min Kang, Seri Lim, Seung Jae Won, Ye Jin Shin, Yun Sook Lim, Byung-Yoon Ahn, Soon B. Hwang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) RNA replication requires cellular factors as well as viral non-structural proteins (NS protein). Using small interfering RNA (siRNA) library screening, we previously identified c-Fos as a host factor involved in HCV propagation. In the present study, we demonstrated that silencing of c-Fos expression resulted in decrease of HCV propagation in cell culture grown HCV (HCVcc)-infected cells; whereas overexpression of c-Fos significantly increased HCV propagation. We further confirmed the positive role of c-Fos in HCV propagation by both HCV-luciferase reporter assay and immunofluorescence analysis. We showed that c-Fos level was upregulated by HCV infection. Furthermore, phorbol 12-myristate 13-acetate (PMA)-induced c-Fos level was synergistically increased by HCV infection. These data suggest that c-Fos acts as a positive regulator of HCV propagation and may contribute to HCV-associated pathogenesis.

Original languageEnglish
Pages (from-to)3236-3244
Number of pages9
JournalFEBS Letters
Volume585
Issue number20
DOIs
Publication statusPublished - 2011 Oct 20

Fingerprint

Viruses
Hepacivirus
Virus Diseases
Viral Nonstructural Proteins
Virus Replication
Luciferases
Cell culture
Small Interfering RNA
Fluorescent Antibody Technique
Assays
Screening
Acetates
Cell Culture Techniques
RNA

Keywords

  • c-Fos
  • Cellular factor
  • Hepatitis C virus
  • Pathogenesis
  • Propagation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Kang, S. M., Lim, S., Won, S. J., Shin, Y. J., Lim, Y. S., Ahn, B-Y., & Hwang, S. B. (2011). C-Fos regulates hepatitis C virus propagation. FEBS Letters, 585(20), 3236-3244. https://doi.org/10.1016/j.febslet.2011.08.041

C-Fos regulates hepatitis C virus propagation. / Kang, Sang Min; Lim, Seri; Won, Seung Jae; Shin, Ye Jin; Lim, Yun Sook; Ahn, Byung-Yoon; Hwang, Soon B.

In: FEBS Letters, Vol. 585, No. 20, 20.10.2011, p. 3236-3244.

Research output: Contribution to journalArticle

Kang, SM, Lim, S, Won, SJ, Shin, YJ, Lim, YS, Ahn, B-Y & Hwang, SB 2011, 'C-Fos regulates hepatitis C virus propagation', FEBS Letters, vol. 585, no. 20, pp. 3236-3244. https://doi.org/10.1016/j.febslet.2011.08.041
Kang SM, Lim S, Won SJ, Shin YJ, Lim YS, Ahn B-Y et al. C-Fos regulates hepatitis C virus propagation. FEBS Letters. 2011 Oct 20;585(20):3236-3244. https://doi.org/10.1016/j.febslet.2011.08.041
Kang, Sang Min ; Lim, Seri ; Won, Seung Jae ; Shin, Ye Jin ; Lim, Yun Sook ; Ahn, Byung-Yoon ; Hwang, Soon B. / C-Fos regulates hepatitis C virus propagation. In: FEBS Letters. 2011 ; Vol. 585, No. 20. pp. 3236-3244.
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