C-reactive protein promotes monocyte chemoattractant protein-1-mediated chemotaxis through upregulating CC chemokine receptor 2 expression in human monocytes

Ki Hoon Han, Kyung Hee Hong, Jae Hyeong Park, Je Sang Ko, Duk Hyun Kang, Kee Joon Choi, Myeong Ki Hong, Seong Wook Park, Seung Jung Park

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Background - Inflammation plays a crucial role in atherosclerosis. An elevated serum C-reactive protein (CRP) level is a strong marker for future atherosclerotic cardiovascular diseases. In addition, recent data suggest that CRP may directly promote atherogenesis. In this study, we investigated whether CRP can directly activate human circulating monocytes. Methods and Results - Incubation of THP-1. monocytes with CRP (10 μg/mL) increased CC chemokine receptor 2 (CCR2) expression at both the protein and transcript levels, which in turn enhanced chemotaxis mediated by monocyte chemoattractant protein-1 (MCP-1) up to 2-fold. The CRP-induced upregulation of CCR2 expression involved binding of CRP to the FcγRI, most notably FcγRI, and phospholipase D1 activation. Serum high-sensitivity CRP levels in 52 normocholesterolemic human subjects were positively correlated with CCR2 surface expression on circulating monocytes (r=0.62, P<0.001) and MCP-1-mediated monocyte chemotaxis (r=0.53, P<0.001). Conclusions - Elevated blood CRP levels may promote accumulation of monocytes in the atherogenic arterial wall by increasing chemotactic activities of monocytes in response to MCP-1.

Original languageEnglish
Pages (from-to)2566-2571
Number of pages6
JournalCirculation
Volume109
Issue number21
DOIs
Publication statusPublished - 2004 Jun 1
Externally publishedYes

Keywords

  • Atherosclerosis
  • C-reactive protein
  • Chemotaxis
  • Monocytes

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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