Caffeic acid phenethyl ester accumulates β-catenin through GSK-3β and participates in proliferation through mTOR in C2C12 cells

Eun Soo Lee, Jung Ok Lee, Soo Kyung Lee, Ji Hae Kim, Jin Hee Jung, Bora Keum, Sun Hwa Park, Hyeon Soo Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aim: The aim of this study is to characterize the roles of caffeic acid phenethyl ester (CAPE) in the skeletal muscle cells. Main methods: We performed immunoblotting assay using various phosphorylation specific antibodies. Key findings: We found that CAPE induces rapid and transient phosphorylation of glycogen synthase kinase (GSK)-3β in a phosphoinositide 3-kinase (PI3K)-dependent manner. CAPE also decreases phosphorylation of β-catenin, ultimately leading to β-catenin accumulation. In addition, we demonstrated that CAPE activated the mammalian target of rapamycin (mTOR)-p70 S6 ribosomal kinase (S6K) and also stimulated extracellular signal-regulated kinase (ERK). The inhibition of mTOR blocked CAPE-induced ERK phosphorylation. Significance: Our results suggest that CAPE may act through β-catenin accumulation via stimulation of GSK-3β and may also participate in cellular proliferation through the mTOR-ERK pathway.

Original languageEnglish
Pages (from-to)755-759
Number of pages5
JournalLife Sciences
Volume84
Issue number21-22
DOIs
Publication statusPublished - 2009 May 22

Keywords

  • Akt
  • CAPE
  • GSK-3β
  • mTOR
  • β-catenin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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