Calcineurin B-like 3 calcium sensor associates with and inhibits 5'-methylthioadenosine nucleosidase 2 in Arabidopsis

Sung Han Ok, Joo Hyuk Cho, Seung Ick Oh, Mi Na Choi, Jae Yeon Ma, Jeong Sheop Shin, Kyung Nam Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Calcineurin B-like (CBL) proteins constitute a unique family of calcium sensor relays in plants. It is well known that CBLs detect the calcium signals elicited by a variety of abiotic stresses and relay the information to a group of serine/threonine protein kinases called CBL-interacting protein kinases (CIPKs). In this study, we found that a few CBL members can also target another group of enzymes 5'-methylthioadenosine nucleosidases (MTANs), which are encoded by two genes in Arabidopsis, AtMTAN1 and AtMTAN2. In the yeast two-hybrid system, AtMTAN1 interacted with multiple CBL members such as CBL2, CBL3 and CBL6, whereas AtMTAN2 associated exclusively with CBL3. We further demonstrated that the CBL3-AtMTAN2 association occurs in a calcium-dependent manner, which results in a significant decrease in the enzyme activity of the AtMTAN2 protein. Taken together, these results clearly indicate that the CBL family can target at least two distinct groups of enzymes (CIPKs and MTANs), conferring an additional level of complexity on the CBL-mediated signaling networks. In addition, our finding also provides a novel molecular mechanism by which calcium signals are transduced to alter metabolite profiles in plants.

Original languageEnglish
Pages (from-to)228-240
Number of pages13
JournalPlant Science
Volume238
DOIs
Publication statusPublished - 2015 Sep 1

Fingerprint

Calcineurin
Arabidopsis
Calcium
calcium
protein kinases
two hybrid system techniques
Protein Kinases
Enzymes
enzymes
abiotic stress
Two-Hybrid System Techniques
proteins
Protein-Serine-Threonine Kinases
enzyme activity
metabolites
yeasts
5'-methylthioadenosine phosphorylase
5'-methylthioadenosine
Proteins
genes

Keywords

  • Arabidopsis
  • Calcium signaling
  • CBL
  • CIPK
  • MTAN

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Agronomy and Crop Science

Cite this

Calcineurin B-like 3 calcium sensor associates with and inhibits 5'-methylthioadenosine nucleosidase 2 in Arabidopsis. / Ok, Sung Han; Cho, Joo Hyuk; Oh, Seung Ick; Choi, Mi Na; Ma, Jae Yeon; Shin, Jeong Sheop; Kim, Kyung Nam.

In: Plant Science, Vol. 238, 01.09.2015, p. 228-240.

Research output: Contribution to journalArticle

Ok, Sung Han ; Cho, Joo Hyuk ; Oh, Seung Ick ; Choi, Mi Na ; Ma, Jae Yeon ; Shin, Jeong Sheop ; Kim, Kyung Nam. / Calcineurin B-like 3 calcium sensor associates with and inhibits 5'-methylthioadenosine nucleosidase 2 in Arabidopsis. In: Plant Science. 2015 ; Vol. 238. pp. 228-240.
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