Calcium imaging in gentamicin ototoxicity: Increased intracellular calcium relates to oxidative stress and late apoptosis

Jiwon Chang, Ji Yun Yang, June Choi, Hak Hyun Jung, Gi Jung Im

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objectives: To estimate intracellular calcium changes in gentamicin (GM) ototoxicity using calcium imaging. To investigate GM-induced physiologic changes in auditory cells including cell viability, apoptosis, and oxidative stress. Methods: Varying concentrations of GM were applied to the HEI-OC1 cochlear cell line. Calcium imaging tracked changes in intracellular calcium concentration during GM cytotoxicity. Cell viability and intracellular reactive oxygen species (ROS) levels also were measured. Results: Little change in calcium levels occurred in HEI-OC1 cells exposed to less than 35. mM GM. However, calcium rose continuously in cells exposed to more than 60. mM GM. With administration of intermediate concentrations of 40 or 50. mM GM, calcium increased variably in different cells, returning to baseline in some cases, or rising continuously in others. Upon increase of GM concentration, intracellular calcium concentration and ROS were increased, and cell viability was decreased due to late apoptosis. Conclusion: This study shows that GM increased intracellular calcium, ROS, and late apoptosis of HEI-OC1 cells derived from cochlear tissue. Increase of intracellular calcium is related to GM-induced apoptosis and oxidative stress. Calcium imaging can be used to determine change of intracellular calcium concentrations and apoptosis in GM ototoxicity.

Original languageEnglish
Pages (from-to)1616-1622
Number of pages7
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume75
Issue number12
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

Gentamicins
Oxidative Stress
Apoptosis
Calcium
Reactive Oxygen Species
Cell Survival
Cochlea
Cell Line

Keywords

  • Apoptosis
  • Calcium
  • Cochlea
  • Fura-2
  • Gentamicins
  • Meniere's disease
  • Reactive oxygen species

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Otorhinolaryngology

Cite this

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title = "Calcium imaging in gentamicin ototoxicity: Increased intracellular calcium relates to oxidative stress and late apoptosis",
abstract = "Objectives: To estimate intracellular calcium changes in gentamicin (GM) ototoxicity using calcium imaging. To investigate GM-induced physiologic changes in auditory cells including cell viability, apoptosis, and oxidative stress. Methods: Varying concentrations of GM were applied to the HEI-OC1 cochlear cell line. Calcium imaging tracked changes in intracellular calcium concentration during GM cytotoxicity. Cell viability and intracellular reactive oxygen species (ROS) levels also were measured. Results: Little change in calcium levels occurred in HEI-OC1 cells exposed to less than 35. mM GM. However, calcium rose continuously in cells exposed to more than 60. mM GM. With administration of intermediate concentrations of 40 or 50. mM GM, calcium increased variably in different cells, returning to baseline in some cases, or rising continuously in others. Upon increase of GM concentration, intracellular calcium concentration and ROS were increased, and cell viability was decreased due to late apoptosis. Conclusion: This study shows that GM increased intracellular calcium, ROS, and late apoptosis of HEI-OC1 cells derived from cochlear tissue. Increase of intracellular calcium is related to GM-induced apoptosis and oxidative stress. Calcium imaging can be used to determine change of intracellular calcium concentrations and apoptosis in GM ototoxicity.",
keywords = "Apoptosis, Calcium, Cochlea, Fura-2, Gentamicins, Meniere's disease, Reactive oxygen species",
author = "Jiwon Chang and Yang, {Ji Yun} and June Choi and Jung, {Hak Hyun} and Im, {Gi Jung}",
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AU - Jung, Hak Hyun

AU - Im, Gi Jung

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AB - Objectives: To estimate intracellular calcium changes in gentamicin (GM) ototoxicity using calcium imaging. To investigate GM-induced physiologic changes in auditory cells including cell viability, apoptosis, and oxidative stress. Methods: Varying concentrations of GM were applied to the HEI-OC1 cochlear cell line. Calcium imaging tracked changes in intracellular calcium concentration during GM cytotoxicity. Cell viability and intracellular reactive oxygen species (ROS) levels also were measured. Results: Little change in calcium levels occurred in HEI-OC1 cells exposed to less than 35. mM GM. However, calcium rose continuously in cells exposed to more than 60. mM GM. With administration of intermediate concentrations of 40 or 50. mM GM, calcium increased variably in different cells, returning to baseline in some cases, or rising continuously in others. Upon increase of GM concentration, intracellular calcium concentration and ROS were increased, and cell viability was decreased due to late apoptosis. Conclusion: This study shows that GM increased intracellular calcium, ROS, and late apoptosis of HEI-OC1 cells derived from cochlear tissue. Increase of intracellular calcium is related to GM-induced apoptosis and oxidative stress. Calcium imaging can be used to determine change of intracellular calcium concentrations and apoptosis in GM ototoxicity.

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KW - Gentamicins

KW - Meniere's disease

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