Candidates for tumor markers of cervical cancer discovered by proteomic analysis

Jae Yun Song, Hyo Sook Bae, Do Hyoung Koo, Jae Kwan Lee, Hak Hyun Jung, Kyu Wan Lee, Nak Woo Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cervical cancer is the second most common gynecological cancer among Korean women. While nationwide screening program has developed, the pathogenesis of cervical cancer is unknown. The aim of this study was to compare the protein expression profilesbetween cervical squamous carcinomas and normal cervical tissues in order to identify proteins that are related to the cancer. Three cervical cancer tissue samples and three normal cervical tissue samples were obtained and protein expression was comparedand was identified in the samples with the use of matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). A total of 20 proteins that showed up-regulated expression in the cervical cancer tissue samples were selected and identified. Seven proteins were matched to allograft inflammatory factor 1 (AIF-1), actine-like protein 2 (ALP2), brain type fatty acid-binding protein (B-FABP), NCK adaptor protein 1 (NCK-1), islet cell autoantigen 1 (ICA69), cationic trypsinogen (PRSS1), and cyclin-dependent kinase 4 (CDK4), but the remaining 13 proteins were unidentifiable. After confirmation by RT-PCR, Western blotting and immunohistochemistry, we found that B-FABP, NCK-1, and CDK4 were related to the pathogenesis of cervical cancer. These proteins are suggested as candidates of new pathological tumor markers for cervical cancer.

Original languageEnglish
Pages (from-to)1479-1485
Number of pages7
JournalJournal of Korean Medical Science
Volume27
Issue number12
DOIs
Publication statusPublished - 2012 Dec 1

    Fingerprint

Keywords

  • Cervical cancer
  • Matrix assisted laser desorption/ionization-time of flight mass spectrometry
  • Two-dimensional polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Medicine(all)

Cite this