Cannabidiol-induced apoptosis is mediated by activation of Noxa in human colorectal cancer cells

Soyeon Jeong, Hye Kyeong Yun, Yoon A. Jeong, Min Jee Jo, Sang Hee Kang, Jung Lim Kim, Dae Yeong Kim, Seong Hye Park, Bo Ram Kim, Yoo Jin Na, Sun Il Lee, Han Do Kim, Dae Hyun Kim, Sang Cheul Oh, Dae Hee Lee

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Cannabidiol (CBD), one of the compounds present in the marijuana plant, has anti-tumor properties, but its mechanism is not well known. This study aimed to evaluate the apoptotic action of CBD in colorectal cancer (CRC) cells, and focused on its effects on the novel pro-apoptotic Noxa-reactive oxygen species (ROS) signaling pathway. CBD experiments were performed using the CRC cell lines HCT116 and DLD-1. CBD induced apoptosis by regulating many pro- and anti-apoptotic proteins, of which Noxa showed significantly higher expression. To understand the relationship between Noxa and CBD-induced apoptosis, Noxa levels were downregulated using siRNA, and the expression of apoptosis markers decreased. After ROS production was blocked, the level of Noxa also decreased, suggesting that ROS is involved in the regulation of Noxa, which along with ROS is a well-known pro-apoptotic signaling agents. As a result, CBD induced apoptosis in a Noxa-and-ROS-dependent manner. Taken together, the results obtained in this study re-demonstrated the effects of CBD treatment in vivo, thus confirming its role as a novel, reliable anticancer drug.

Original languageEnglish
Pages (from-to)12-23
Number of pages12
JournalCancer Letters
Volume447
DOIs
Publication statusPublished - 2019 Apr 10

Keywords

  • Apoptotic cell death
  • Bcl-2 protein family
  • Colon cancer
  • Marijuana extract
  • ROS

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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