Capped poly(A) leaders of variable lengths at the 5' ends of vaccinia virus late mRNAs

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Abstract

Evidence for capped poly(A) leaders of variable lengths located immediately upstream of the translation initiation codon was obtained by direct analyses of a major late mRNA species. A decapping-recapping method was used to specifically substitute a radioactively labeled phosphate for an unlabeled one within the cap structure. RNase H-susceptible sites were made by hybridizing synthetic oligodeoxyribonucleotides to the mRNA encoding a late major structural protein of 11 kilodaltons. Sequences of the type m7G(5')pppAmp (Ap)(n)UpG..., where n varies from a few to more than 40 nucleotides, were deduced by analysis of the length and sequence of RNase H, RNase, T1, and RNase U2 digestion products.

Original languageEnglish
Pages (from-to)226-232
Number of pages7
JournalJournal of Virology
Volume63
Issue number1
Publication statusPublished - 1989 Jan 1
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

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