Capped poly(A) leaders of variable lengths at the 5' ends of vaccinia virus late mRNAs

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Evidence for capped poly(A) leaders of variable lengths located immediately upstream of the translation initiation codon was obtained by direct analyses of a major late mRNA species. A decapping-recapping method was used to specifically substitute a radioactively labeled phosphate for an unlabeled one within the cap structure. RNase H-susceptible sites were made by hybridizing synthetic oligodeoxyribonucleotides to the mRNA encoding a late major structural protein of 11 kilodaltons. Sequences of the type m7G(5')pppAmp (Ap)(n)UpG..., where n varies from a few to more than 40 nucleotides, were deduced by analysis of the length and sequence of RNase H, RNase, T1, and RNase U2 digestion products.

Original languageEnglish
Pages (from-to)226-232
Number of pages7
JournalJournal of Virology
Volume63
Issue number1
Publication statusPublished - 1989 Jan 1
Externally publishedYes

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Ribonuclease H
Poly A
Vaccinia virus
Ribonuclease T1
Messenger RNA
Initiator Codon
Oligodeoxyribonucleotides
Sequence Analysis
Digestion
Nucleotides
Phosphates
Proteins
ribonuclease U2

ASJC Scopus subject areas

  • Immunology

Cite this

Capped poly(A) leaders of variable lengths at the 5' ends of vaccinia virus late mRNAs. / Ahn, Byung-Yoon; Moss, B.

In: Journal of Virology, Vol. 63, No. 1, 01.01.1989, p. 226-232.

Research output: Contribution to journalArticle

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