Evidence for capped poly(A) leaders of variable lengths located immediately upstream of the translation initiation codon was obtained by direct analyses of a major late mRNA species. A decapping-recapping method was used to specifically substitute a radioactively labeled phosphate for an unlabeled one within the cap structure. RNase H-susceptible sites were made by hybridizing synthetic oligodeoxyribonucleotides to the mRNA encoding a late major structural protein of 11 kilodaltons. Sequences of the type m7G(5')pppAmp (Ap)(n)UpG..., where n varies from a few to more than 40 nucleotides, were deduced by analysis of the length and sequence of RNase H, RNase, T1, and RNase U2 digestion products.
ASJC Scopus subject areas
- Insect Science