TY - JOUR
T1 - Capsicum annuum basic transcription factor 3 (CaBtf3) regulates transcription of pathogenesis-related genes during hypersensitive response upon Tobacco mosaic virus infection
AU - Huh, Sung Un
AU - Kim, Ki Jeong
AU - Paek, Kyung Hee
N1 - Funding Information:
This work was supported by the Science Research Center-Engineering Research Center Program (Plant Signaling Network Research Center) of the Ministry of Education, Science and Technology (R11-2003-008-02001-0), the Mid-career Researcher-Program through a National Research Foundation grant funded by the MEST (2009-0085565), and the Wujangchoon Project (PJ007850) from the Rural Development Administration, Republic of Korea.
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012/1/13
Y1 - 2012/1/13
N2 - Hypersensitive response (HR) cell death upon plant virus infection is an excellent plant strategy for inhibiting viral movement and obtaining systemic acquired resistance (SAR) against further infection. Various host factors are involved in these HR processes, either directly as viral resistance proteins or indirectly. We characterized a gene encoding the CaBtf3 [β-nascent polypeptide-associated complex (NAC) subunit] of NAC from the hot pepper plant. NAC contacts nascent polypeptides to prevent aggregation and degradation of newly synthesized proteins by controlling cotranslational protein folding. CaBtf3 protein fused to green fluorescent protein predominantly localized to the nucleus. Silencing phenotype of CaBtf3 upon the Tobacco mosaic virus (TMV)-P0 inoculation exhibited reduced HR cell death and decreased expression of some HR-associated genes, but increased TMV coat protein levels compared with TRV2 control plants. Furthermore, silencing of NbBtf3, a highly homologous gene of CaBtf3, also led to the reduced Bax- and Pto-mediated cell death. The results indicate that CaBtf3 might be involved in HR cell death and could function as a transcription factor in the nucleus by transcriptional regulation of HR-related gene expression.
AB - Hypersensitive response (HR) cell death upon plant virus infection is an excellent plant strategy for inhibiting viral movement and obtaining systemic acquired resistance (SAR) against further infection. Various host factors are involved in these HR processes, either directly as viral resistance proteins or indirectly. We characterized a gene encoding the CaBtf3 [β-nascent polypeptide-associated complex (NAC) subunit] of NAC from the hot pepper plant. NAC contacts nascent polypeptides to prevent aggregation and degradation of newly synthesized proteins by controlling cotranslational protein folding. CaBtf3 protein fused to green fluorescent protein predominantly localized to the nucleus. Silencing phenotype of CaBtf3 upon the Tobacco mosaic virus (TMV)-P0 inoculation exhibited reduced HR cell death and decreased expression of some HR-associated genes, but increased TMV coat protein levels compared with TRV2 control plants. Furthermore, silencing of NbBtf3, a highly homologous gene of CaBtf3, also led to the reduced Bax- and Pto-mediated cell death. The results indicate that CaBtf3 might be involved in HR cell death and could function as a transcription factor in the nucleus by transcriptional regulation of HR-related gene expression.
KW - Capsicum annuum basic transcription factor 3 (CaBtf3)
KW - Hypersensitive response (HR)
KW - Nascent polypeptide-associated complex (NAC)
KW - Tobacco mosaic virus (TMV)-P
KW - Virus-induced gene silencing (VIGS)
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U2 - 10.1016/j.bbrc.2011.12.074
DO - 10.1016/j.bbrc.2011.12.074
M3 - Article
C2 - 22209846
AN - SCOPUS:84855826721
VL - 417
SP - 910
EP - 917
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -