Abstract
Hypersensitive response (HR) cell death upon plant virus infection is an excellent plant strategy for inhibiting viral movement and obtaining systemic acquired resistance (SAR) against further infection. Various host factors are involved in these HR processes, either directly as viral resistance proteins or indirectly. We characterized a gene encoding the CaBtf3 [β-nascent polypeptide-associated complex (NAC) subunit] of NAC from the hot pepper plant. NAC contacts nascent polypeptides to prevent aggregation and degradation of newly synthesized proteins by controlling cotranslational protein folding. CaBtf3 protein fused to green fluorescent protein predominantly localized to the nucleus. Silencing phenotype of CaBtf3 upon the Tobacco mosaic virus (TMV)-P0 inoculation exhibited reduced HR cell death and decreased expression of some HR-associated genes, but increased TMV coat protein levels compared with TRV2 control plants. Furthermore, silencing of NbBtf3, a highly homologous gene of CaBtf3, also led to the reduced Bax- and Pto-mediated cell death. The results indicate that CaBtf3 might be involved in HR cell death and could function as a transcription factor in the nucleus by transcriptional regulation of HR-related gene expression.
| Original language | English |
|---|---|
| Pages (from-to) | 910-917 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 417 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 Jan 13 |
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Keywords
- Capsicum annuum basic transcription factor 3 (CaBtf3)
- Hypersensitive response (HR)
- Nascent polypeptide-associated complex (NAC)
- Tobacco mosaic virus (TMV)-P
- Virus-induced gene silencing (VIGS)
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Molecular Biology
Cite this
Capsicum annuum basic transcription factor 3 (CaBtf3) regulates transcription of pathogenesis-related genes during hypersensitive response upon Tobacco mosaic virus infection. / Huh, Sung Un; Kim, Ki Jeong; Paek, Kyung Hee.
In: Biochemical and Biophysical Research Communications, Vol. 417, No. 2, 13.01.2012, p. 910-917.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Capsicum annuum basic transcription factor 3 (CaBtf3) regulates transcription of pathogenesis-related genes during hypersensitive response upon Tobacco mosaic virus infection
AU - Huh, Sung Un
AU - Kim, Ki Jeong
AU - Paek, Kyung Hee
PY - 2012/1/13
Y1 - 2012/1/13
N2 - Hypersensitive response (HR) cell death upon plant virus infection is an excellent plant strategy for inhibiting viral movement and obtaining systemic acquired resistance (SAR) against further infection. Various host factors are involved in these HR processes, either directly as viral resistance proteins or indirectly. We characterized a gene encoding the CaBtf3 [β-nascent polypeptide-associated complex (NAC) subunit] of NAC from the hot pepper plant. NAC contacts nascent polypeptides to prevent aggregation and degradation of newly synthesized proteins by controlling cotranslational protein folding. CaBtf3 protein fused to green fluorescent protein predominantly localized to the nucleus. Silencing phenotype of CaBtf3 upon the Tobacco mosaic virus (TMV)-P0 inoculation exhibited reduced HR cell death and decreased expression of some HR-associated genes, but increased TMV coat protein levels compared with TRV2 control plants. Furthermore, silencing of NbBtf3, a highly homologous gene of CaBtf3, also led to the reduced Bax- and Pto-mediated cell death. The results indicate that CaBtf3 might be involved in HR cell death and could function as a transcription factor in the nucleus by transcriptional regulation of HR-related gene expression.
AB - Hypersensitive response (HR) cell death upon plant virus infection is an excellent plant strategy for inhibiting viral movement and obtaining systemic acquired resistance (SAR) against further infection. Various host factors are involved in these HR processes, either directly as viral resistance proteins or indirectly. We characterized a gene encoding the CaBtf3 [β-nascent polypeptide-associated complex (NAC) subunit] of NAC from the hot pepper plant. NAC contacts nascent polypeptides to prevent aggregation and degradation of newly synthesized proteins by controlling cotranslational protein folding. CaBtf3 protein fused to green fluorescent protein predominantly localized to the nucleus. Silencing phenotype of CaBtf3 upon the Tobacco mosaic virus (TMV)-P0 inoculation exhibited reduced HR cell death and decreased expression of some HR-associated genes, but increased TMV coat protein levels compared with TRV2 control plants. Furthermore, silencing of NbBtf3, a highly homologous gene of CaBtf3, also led to the reduced Bax- and Pto-mediated cell death. The results indicate that CaBtf3 might be involved in HR cell death and could function as a transcription factor in the nucleus by transcriptional regulation of HR-related gene expression.
KW - Capsicum annuum basic transcription factor 3 (CaBtf3)
KW - Hypersensitive response (HR)
KW - Nascent polypeptide-associated complex (NAC)
KW - Tobacco mosaic virus (TMV)-P
KW - Virus-induced gene silencing (VIGS)
UR - http://www.scopus.com/inward/record.url?scp=84855826721&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84855826721&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2011.12.074
DO - 10.1016/j.bbrc.2011.12.074
M3 - Article
C2 - 22209846
AN - SCOPUS:84855826721
VL - 417
SP - 910
EP - 917
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 2
ER -