Capsicum annuum WRKYb transcription factor that binds to the CaPR-10 promoter functions as a positive regulator in innate immunity upon TMV infection

Jee Hyuck Lim, Chang Jin Park, Sung Un Huh, La Mee Choi, Gil Je Lee, Young Jin Kim, Kyung Hee Paek

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In plant, some WRKY transcription factors are known to play an important role in the transcriptional reprogramming associated with the immune response. By using WRKY-domain-specific differential display procedure, we isolated CaWRKYb gene, which is rapidly induced during an incompatible interaction between hot pepper and Tobacco mosaic virus (TMV) pathotype P0 infection. The recombinant CaWRKYb bound to the W box-containing CaPR-10 promoter probes efficiently and the specificity of binding was confirmed by mutant study and competition with cold oligonucleotides. Also, in GUS reporter activity assay using Arabidopsis protoplasts with the CaPR-10 promoter, GUS activity was increased in the presence of CaWRKYb. And CaWRKYb-knockdown plant showed reduced number of hypersensitive response local lesions upon TMV-P0 infection. Furthermore, CaWRKYb-knockdown plant exhibited compromised resistance to TMV-P0 by accumulating more TMV, apparently through decreased expression of CaPR-10, CaPR-1, and CaPR-5. These results suggest that CaWRKYb is involved as a positive transcription factor in defense-related signal transduction pathways in hot pepper.

Original languageEnglish
Pages (from-to)613-619
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume411
Issue number3
DOIs
Publication statusPublished - 2011 Aug 5

Fingerprint

Tobacco Mosaic Virus
Capsicum
Tobacco
Virus Diseases
Viruses
Innate Immunity
Transcription Factors
Signal transduction
Protoplasts
Arabidopsis
Oligonucleotides
Assays
Signal Transduction
Genes
Display devices
Infection

Keywords

  • CaPR-10 promoter
  • Hot pepper (Capsicum annuum L.)
  • Tobacco mosaic virus
  • VIGS
  • WRKY transcription factor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Capsicum annuum WRKYb transcription factor that binds to the CaPR-10 promoter functions as a positive regulator in innate immunity upon TMV infection. / Lim, Jee Hyuck; Park, Chang Jin; Huh, Sung Un; Choi, La Mee; Lee, Gil Je; Kim, Young Jin; Paek, Kyung Hee.

In: Biochemical and Biophysical Research Communications, Vol. 411, No. 3, 05.08.2011, p. 613-619.

Research output: Contribution to journalArticle

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