Carbon nanotube based electrochemical immunosensors for high-sensitive detection of E. coli

Jun Yong Lee, Eun Jin Park, Nam Ki Minp, James Jungho Pak, Cheol Jin Lee, Min Ja Kim, Soo Won Kim, Suk In Hong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

This paper outlines the fabrication and characterization of an immunosensor based on electrodchemical, biological, and nano-material techniques to achieve high sensitive detection of Escherichia coli (E.coli). The working electrode consists of E.coli antibody/antigen/horseradish peroxidase(HRP) labeled antibody immobilized on plasma-functionalized multiwalled carbon nanotube (pf-MWCNT) film. Immunosensors were characterized by cyclic voltammetry using 10 mM K 3Fe(CN)6/ 3 M KCl and 3,3′,5,5′- tetramethylbenzidine (TMB). This immunosensor shows very high sensitivity to E. coli O157:H7 and detection limit as low as 0.031 ng/ml. This is 100 times lower than that achievable with standard spectrophotometric enzyme-linked immunosorbent assay (ELISA) using the same immunochemicals.

Original languageEnglish
Title of host publicationIEEE Sensors 2009 Conference - SENSORS 2009
Pages1176-1179
Number of pages4
DOIs
Publication statusPublished - 2009
EventIEEE Sensors 2009 Conference - SENSORS 2009 - Christchurch, New Zealand
Duration: 2009 Oct 252009 Oct 28

Publication series

NameProceedings of IEEE Sensors

Other

OtherIEEE Sensors 2009 Conference - SENSORS 2009
CountryNew Zealand
CityChristchurch
Period09/10/2509/10/28

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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    Lee, J. Y., Park, E. J., Minp, N. K., Pak, J. J., Lee, C. J., Kim, M. J., Kim, S. W., & Hong, S. I. (2009). Carbon nanotube based electrochemical immunosensors for high-sensitive detection of E. coli. In IEEE Sensors 2009 Conference - SENSORS 2009 (pp. 1176-1179). [5398352] (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2009.5398352