Carbon nanotube based electrochemical immunosensors for high-sensitive detection of E. coli

Jun Yong Lee, Eun Jin Park, Nam Ki Minp, James Jungho Pak, Cheol Jin Lee, Ja Kim Min, Soo-Won Kim, Suk In Hong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

This paper outlines the fabrication and characterization of an immunosensor based on electrodchemical, biological, and nano-material techniques to achieve high sensitive detection of Escherichia coli (E.coli). The working electrode consists of E.coli antibody/antigen/horseradish peroxidase(HRP) labeled antibody immobilized on plasma-functionalized multiwalled carbon nanotube (pf-MWCNT) film. Immunosensors were characterized by cyclic voltammetry using 10 mM K 3Fe(CN)6/ 3 M KCl and 3,3′,5,5′- tetramethylbenzidine (TMB). This immunosensor shows very high sensitivity to E. coli O157:H7 and detection limit as low as 0.031 ng/ml. This is 100 times lower than that achievable with standard spectrophotometric enzyme-linked immunosorbent assay (ELISA) using the same immunochemicals.

Original languageEnglish
Title of host publicationProceedings of IEEE Sensors
Pages1176-1179
Number of pages4
DOIs
Publication statusPublished - 2009 Dec 1
EventIEEE Sensors 2009 Conference - SENSORS 2009 - Christchurch, New Zealand
Duration: 2009 Oct 252009 Oct 28

Other

OtherIEEE Sensors 2009 Conference - SENSORS 2009
CountryNew Zealand
CityChristchurch
Period09/10/2509/10/28

    Fingerprint

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this