Carbon nanotube based electrochemical immunosensors for high-sensitive detection of E. coli

Jun Yong Lee, Eun Jin Park, Nam Ki Minp, James Jungho Pak, Cheol Jin Lee, Ja Kim Min, Soo-Won Kim, Suk In Hong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

This paper outlines the fabrication and characterization of an immunosensor based on electrodchemical, biological, and nano-material techniques to achieve high sensitive detection of Escherichia coli (E.coli). The working electrode consists of E.coli antibody/antigen/horseradish peroxidase(HRP) labeled antibody immobilized on plasma-functionalized multiwalled carbon nanotube (pf-MWCNT) film. Immunosensors were characterized by cyclic voltammetry using 10 mM K 3Fe(CN)6/ 3 M KCl and 3,3′,5,5′- tetramethylbenzidine (TMB). This immunosensor shows very high sensitivity to E. coli O157:H7 and detection limit as low as 0.031 ng/ml. This is 100 times lower than that achievable with standard spectrophotometric enzyme-linked immunosorbent assay (ELISA) using the same immunochemicals.

Original languageEnglish
Title of host publicationProceedings of IEEE Sensors
Pages1176-1179
Number of pages4
DOIs
Publication statusPublished - 2009 Dec 1
EventIEEE Sensors 2009 Conference - SENSORS 2009 - Christchurch, New Zealand
Duration: 2009 Oct 252009 Oct 28

Other

OtherIEEE Sensors 2009 Conference - SENSORS 2009
CountryNew Zealand
CityChristchurch
Period09/10/2509/10/28

Fingerprint

Immunosensors
Escherichia coli
Carbon nanotubes
Antibodies
Multiwalled carbon nanotubes (MWCN)
Antigens
Cyclic voltammetry
Assays
Enzymes
Plasmas
Fabrication
Electrodes

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Lee, J. Y., Park, E. J., Minp, N. K., Pak, J. J., Lee, C. J., Min, J. K., ... Hong, S. I. (2009). Carbon nanotube based electrochemical immunosensors for high-sensitive detection of E. coli. In Proceedings of IEEE Sensors (pp. 1176-1179). [5398352] https://doi.org/10.1109/ICSENS.2009.5398352

Carbon nanotube based electrochemical immunosensors for high-sensitive detection of E. coli. / Lee, Jun Yong; Park, Eun Jin; Minp, Nam Ki; Pak, James Jungho; Lee, Cheol Jin; Min, Ja Kim; Kim, Soo-Won; Hong, Suk In.

Proceedings of IEEE Sensors. 2009. p. 1176-1179 5398352.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lee, JY, Park, EJ, Minp, NK, Pak, JJ, Lee, CJ, Min, JK, Kim, S-W & Hong, SI 2009, Carbon nanotube based electrochemical immunosensors for high-sensitive detection of E. coli. in Proceedings of IEEE Sensors., 5398352, pp. 1176-1179, IEEE Sensors 2009 Conference - SENSORS 2009, Christchurch, New Zealand, 09/10/25. https://doi.org/10.1109/ICSENS.2009.5398352
Lee, Jun Yong ; Park, Eun Jin ; Minp, Nam Ki ; Pak, James Jungho ; Lee, Cheol Jin ; Min, Ja Kim ; Kim, Soo-Won ; Hong, Suk In. / Carbon nanotube based electrochemical immunosensors for high-sensitive detection of E. coli. Proceedings of IEEE Sensors. 2009. pp. 1176-1179
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