Abstract
Background Long-term clinical implications of embryonic stem cell markers such as Oct4 and Nanog have not been investigated in ST-elevation myocardial infarction (STEMI) patients. The aim of this study was to investigate the effects of early peripheral mobilization of stem cells with Oct4 and Nanog gene expression on major adverse cardiovascular events (MACEs) in patients with STEMI during a 4-year follow-up. Methods Peripheral blood mononuclear cells (PBMCs) were isolated on days 0, 1 and 7 from patients with STEMI (n = 40) and healthy controls (n = 20). The numbers of CD34 +, CD117 +, CD133 + and c-met + stem cells were measured by flow-cytometry. Oct4 and Nanog gene expressions were analyzed by real-time PCR. MACEs such as non-fatal MI, death, stroke, target lesion and revascularization were observed. Results MACEs were significantly lower in patients with Oct4 gene expression ≥ 1.13 and Nanog gene expression ≥ 1.20 at admission. The numbers of CD34 +, CD117 +, CD133 + and c-met + cells within 7 days after STEMI did not show significant differences in patients with or without MACE. Level of anti-inflammatory marker such as IL-10 was significantly higher within 7 days following STEMI in patients without MACE. Inflammatory and angiogenic markers such as CRP, IL-6, SCF, SDF-1α, and VEGF did not show significant differences in patients with or without MACE. Conclusion mRNA levels of pluripotent embryonic stem cell markers such as Oct4 and Nanog were significantly higher in STEMI patients without MACEs during a 4-year follow-up. Baseline Oct4 and Nanog gene expression levels could be used as predictors of MACE in STEMI patients.
| Original language | English |
|---|---|
| Pages (from-to) | 2533-2539 |
| Number of pages | 7 |
| Journal | International Journal of Cardiology |
| Volume | 168 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2013 Oct 3 |
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Keywords
- Circulating stem cells
- Cytokines
- Myocardial infarction
- Pluripotent embryonic stem cell markers
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Cardiovascular event rates in patients with ST-elevation myocardial infarction were lower with early increases in mobilization of Oct4 highNanoghigh stem cells into the peripheral circulation during a 4-year follow-up. / Yu, Cheol Woong; Choi, Seung Cheol; Hong, Soon Jun; Choi, Ji Hyun; Park, Chi Yeon; Kim, Jong Ho; Park, Jae Hyoung; Ahn, Chul Min; Lim, Do-Sun.
In: International Journal of Cardiology, Vol. 168, No. 3, 03.10.2013, p. 2533-2539.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cardiovascular event rates in patients with ST-elevation myocardial infarction were lower with early increases in mobilization of Oct4 highNanoghigh stem cells into the peripheral circulation during a 4-year follow-up
AU - Yu, Cheol Woong
AU - Choi, Seung Cheol
AU - Hong, Soon Jun
AU - Choi, Ji Hyun
AU - Park, Chi Yeon
AU - Kim, Jong Ho
AU - Park, Jae Hyoung
AU - Ahn, Chul Min
AU - Lim, Do-Sun
PY - 2013/10/3
Y1 - 2013/10/3
N2 - Background Long-term clinical implications of embryonic stem cell markers such as Oct4 and Nanog have not been investigated in ST-elevation myocardial infarction (STEMI) patients. The aim of this study was to investigate the effects of early peripheral mobilization of stem cells with Oct4 and Nanog gene expression on major adverse cardiovascular events (MACEs) in patients with STEMI during a 4-year follow-up. Methods Peripheral blood mononuclear cells (PBMCs) were isolated on days 0, 1 and 7 from patients with STEMI (n = 40) and healthy controls (n = 20). The numbers of CD34 +, CD117 +, CD133 + and c-met + stem cells were measured by flow-cytometry. Oct4 and Nanog gene expressions were analyzed by real-time PCR. MACEs such as non-fatal MI, death, stroke, target lesion and revascularization were observed. Results MACEs were significantly lower in patients with Oct4 gene expression ≥ 1.13 and Nanog gene expression ≥ 1.20 at admission. The numbers of CD34 +, CD117 +, CD133 + and c-met + cells within 7 days after STEMI did not show significant differences in patients with or without MACE. Level of anti-inflammatory marker such as IL-10 was significantly higher within 7 days following STEMI in patients without MACE. Inflammatory and angiogenic markers such as CRP, IL-6, SCF, SDF-1α, and VEGF did not show significant differences in patients with or without MACE. Conclusion mRNA levels of pluripotent embryonic stem cell markers such as Oct4 and Nanog were significantly higher in STEMI patients without MACEs during a 4-year follow-up. Baseline Oct4 and Nanog gene expression levels could be used as predictors of MACE in STEMI patients.
AB - Background Long-term clinical implications of embryonic stem cell markers such as Oct4 and Nanog have not been investigated in ST-elevation myocardial infarction (STEMI) patients. The aim of this study was to investigate the effects of early peripheral mobilization of stem cells with Oct4 and Nanog gene expression on major adverse cardiovascular events (MACEs) in patients with STEMI during a 4-year follow-up. Methods Peripheral blood mononuclear cells (PBMCs) were isolated on days 0, 1 and 7 from patients with STEMI (n = 40) and healthy controls (n = 20). The numbers of CD34 +, CD117 +, CD133 + and c-met + stem cells were measured by flow-cytometry. Oct4 and Nanog gene expressions were analyzed by real-time PCR. MACEs such as non-fatal MI, death, stroke, target lesion and revascularization were observed. Results MACEs were significantly lower in patients with Oct4 gene expression ≥ 1.13 and Nanog gene expression ≥ 1.20 at admission. The numbers of CD34 +, CD117 +, CD133 + and c-met + cells within 7 days after STEMI did not show significant differences in patients with or without MACE. Level of anti-inflammatory marker such as IL-10 was significantly higher within 7 days following STEMI in patients without MACE. Inflammatory and angiogenic markers such as CRP, IL-6, SCF, SDF-1α, and VEGF did not show significant differences in patients with or without MACE. Conclusion mRNA levels of pluripotent embryonic stem cell markers such as Oct4 and Nanog were significantly higher in STEMI patients without MACEs during a 4-year follow-up. Baseline Oct4 and Nanog gene expression levels could be used as predictors of MACE in STEMI patients.
KW - Circulating stem cells
KW - Cytokines
KW - Myocardial infarction
KW - Pluripotent embryonic stem cell markers
UR - http://www.scopus.com/inward/record.url?scp=84885661891&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885661891&partnerID=8YFLogxK
U2 - 10.1016/j.ijcard.2013.03.060
DO - 10.1016/j.ijcard.2013.03.060
M3 - Article
VL - 168
SP - 2533
EP - 2539
JO - International Journal of Cardiology
JF - International Journal of Cardiology
SN - 0167-5273
IS - 3
ER -