Cardiovascular event rates in patients with ST-elevation myocardial infarction were lower with early increases in mobilization of Oct4 highNanoghigh stem cells into the peripheral circulation during a 4-year follow-up

Cheol Woong Yu, Seung Cheol Choi, Soon Jun Hong, Ji Hyun Choi, Chi Yeon Park, Jong Ho Kim, Jae Hyoung Park, Chul Min Ahn, Do-Sun Lim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background Long-term clinical implications of embryonic stem cell markers such as Oct4 and Nanog have not been investigated in ST-elevation myocardial infarction (STEMI) patients. The aim of this study was to investigate the effects of early peripheral mobilization of stem cells with Oct4 and Nanog gene expression on major adverse cardiovascular events (MACEs) in patients with STEMI during a 4-year follow-up. Methods Peripheral blood mononuclear cells (PBMCs) were isolated on days 0, 1 and 7 from patients with STEMI (n = 40) and healthy controls (n = 20). The numbers of CD34 +, CD117 +, CD133 + and c-met + stem cells were measured by flow-cytometry. Oct4 and Nanog gene expressions were analyzed by real-time PCR. MACEs such as non-fatal MI, death, stroke, target lesion and revascularization were observed. Results MACEs were significantly lower in patients with Oct4 gene expression ≥ 1.13 and Nanog gene expression ≥ 1.20 at admission. The numbers of CD34 +, CD117 +, CD133 + and c-met + cells within 7 days after STEMI did not show significant differences in patients with or without MACE. Level of anti-inflammatory marker such as IL-10 was significantly higher within 7 days following STEMI in patients without MACE. Inflammatory and angiogenic markers such as CRP, IL-6, SCF, SDF-1α, and VEGF did not show significant differences in patients with or without MACE. Conclusion mRNA levels of pluripotent embryonic stem cell markers such as Oct4 and Nanog were significantly higher in STEMI patients without MACEs during a 4-year follow-up. Baseline Oct4 and Nanog gene expression levels could be used as predictors of MACE in STEMI patients.

Original languageEnglish
Pages (from-to)2533-2539
Number of pages7
JournalInternational Journal of Cardiology
Volume168
Issue number3
DOIs
Publication statusPublished - 2013 Oct 3

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Gene Expression
Embryonic Stem Cells
ST Elevation Myocardial Infarction
Peripheral Blood Stem Cells
Early Ambulation
Pluripotent Stem Cells
Interleukin-10
Vascular Endothelial Growth Factor A
Real-Time Polymerase Chain Reaction
Interleukin-6
Blood Cells
Flow Cytometry
Anti-Inflammatory Agents
Stem Cells
Stroke
Messenger RNA

Keywords

  • Circulating stem cells
  • Cytokines
  • Myocardial infarction
  • Pluripotent embryonic stem cell markers

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Cardiovascular event rates in patients with ST-elevation myocardial infarction were lower with early increases in mobilization of Oct4 highNanoghigh stem cells into the peripheral circulation during a 4-year follow-up. / Yu, Cheol Woong; Choi, Seung Cheol; Hong, Soon Jun; Choi, Ji Hyun; Park, Chi Yeon; Kim, Jong Ho; Park, Jae Hyoung; Ahn, Chul Min; Lim, Do-Sun.

In: International Journal of Cardiology, Vol. 168, No. 3, 03.10.2013, p. 2533-2539.

Research output: Contribution to journalArticle

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title = "Cardiovascular event rates in patients with ST-elevation myocardial infarction were lower with early increases in mobilization of Oct4 highNanoghigh stem cells into the peripheral circulation during a 4-year follow-up",
abstract = "Background Long-term clinical implications of embryonic stem cell markers such as Oct4 and Nanog have not been investigated in ST-elevation myocardial infarction (STEMI) patients. The aim of this study was to investigate the effects of early peripheral mobilization of stem cells with Oct4 and Nanog gene expression on major adverse cardiovascular events (MACEs) in patients with STEMI during a 4-year follow-up. Methods Peripheral blood mononuclear cells (PBMCs) were isolated on days 0, 1 and 7 from patients with STEMI (n = 40) and healthy controls (n = 20). The numbers of CD34 +, CD117 +, CD133 + and c-met + stem cells were measured by flow-cytometry. Oct4 and Nanog gene expressions were analyzed by real-time PCR. MACEs such as non-fatal MI, death, stroke, target lesion and revascularization were observed. Results MACEs were significantly lower in patients with Oct4 gene expression ≥ 1.13 and Nanog gene expression ≥ 1.20 at admission. The numbers of CD34 +, CD117 +, CD133 + and c-met + cells within 7 days after STEMI did not show significant differences in patients with or without MACE. Level of anti-inflammatory marker such as IL-10 was significantly higher within 7 days following STEMI in patients without MACE. Inflammatory and angiogenic markers such as CRP, IL-6, SCF, SDF-1α, and VEGF did not show significant differences in patients with or without MACE. Conclusion mRNA levels of pluripotent embryonic stem cell markers such as Oct4 and Nanog were significantly higher in STEMI patients without MACEs during a 4-year follow-up. Baseline Oct4 and Nanog gene expression levels could be used as predictors of MACE in STEMI patients.",
keywords = "Circulating stem cells, Cytokines, Myocardial infarction, Pluripotent embryonic stem cell markers",
author = "Yu, {Cheol Woong} and Choi, {Seung Cheol} and Hong, {Soon Jun} and Choi, {Ji Hyun} and Park, {Chi Yeon} and Kim, {Jong Ho} and Park, {Jae Hyoung} and Ahn, {Chul Min} and Do-Sun Lim",
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T1 - Cardiovascular event rates in patients with ST-elevation myocardial infarction were lower with early increases in mobilization of Oct4 highNanoghigh stem cells into the peripheral circulation during a 4-year follow-up

AU - Yu, Cheol Woong

AU - Choi, Seung Cheol

AU - Hong, Soon Jun

AU - Choi, Ji Hyun

AU - Park, Chi Yeon

AU - Kim, Jong Ho

AU - Park, Jae Hyoung

AU - Ahn, Chul Min

AU - Lim, Do-Sun

PY - 2013/10/3

Y1 - 2013/10/3

N2 - Background Long-term clinical implications of embryonic stem cell markers such as Oct4 and Nanog have not been investigated in ST-elevation myocardial infarction (STEMI) patients. The aim of this study was to investigate the effects of early peripheral mobilization of stem cells with Oct4 and Nanog gene expression on major adverse cardiovascular events (MACEs) in patients with STEMI during a 4-year follow-up. Methods Peripheral blood mononuclear cells (PBMCs) were isolated on days 0, 1 and 7 from patients with STEMI (n = 40) and healthy controls (n = 20). The numbers of CD34 +, CD117 +, CD133 + and c-met + stem cells were measured by flow-cytometry. Oct4 and Nanog gene expressions were analyzed by real-time PCR. MACEs such as non-fatal MI, death, stroke, target lesion and revascularization were observed. Results MACEs were significantly lower in patients with Oct4 gene expression ≥ 1.13 and Nanog gene expression ≥ 1.20 at admission. The numbers of CD34 +, CD117 +, CD133 + and c-met + cells within 7 days after STEMI did not show significant differences in patients with or without MACE. Level of anti-inflammatory marker such as IL-10 was significantly higher within 7 days following STEMI in patients without MACE. Inflammatory and angiogenic markers such as CRP, IL-6, SCF, SDF-1α, and VEGF did not show significant differences in patients with or without MACE. Conclusion mRNA levels of pluripotent embryonic stem cell markers such as Oct4 and Nanog were significantly higher in STEMI patients without MACEs during a 4-year follow-up. Baseline Oct4 and Nanog gene expression levels could be used as predictors of MACE in STEMI patients.

AB - Background Long-term clinical implications of embryonic stem cell markers such as Oct4 and Nanog have not been investigated in ST-elevation myocardial infarction (STEMI) patients. The aim of this study was to investigate the effects of early peripheral mobilization of stem cells with Oct4 and Nanog gene expression on major adverse cardiovascular events (MACEs) in patients with STEMI during a 4-year follow-up. Methods Peripheral blood mononuclear cells (PBMCs) were isolated on days 0, 1 and 7 from patients with STEMI (n = 40) and healthy controls (n = 20). The numbers of CD34 +, CD117 +, CD133 + and c-met + stem cells were measured by flow-cytometry. Oct4 and Nanog gene expressions were analyzed by real-time PCR. MACEs such as non-fatal MI, death, stroke, target lesion and revascularization were observed. Results MACEs were significantly lower in patients with Oct4 gene expression ≥ 1.13 and Nanog gene expression ≥ 1.20 at admission. The numbers of CD34 +, CD117 +, CD133 + and c-met + cells within 7 days after STEMI did not show significant differences in patients with or without MACE. Level of anti-inflammatory marker such as IL-10 was significantly higher within 7 days following STEMI in patients without MACE. Inflammatory and angiogenic markers such as CRP, IL-6, SCF, SDF-1α, and VEGF did not show significant differences in patients with or without MACE. Conclusion mRNA levels of pluripotent embryonic stem cell markers such as Oct4 and Nanog were significantly higher in STEMI patients without MACEs during a 4-year follow-up. Baseline Oct4 and Nanog gene expression levels could be used as predictors of MACE in STEMI patients.

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KW - Cytokines

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