Abstract
It is known that complex loading is involved in the development and maintenance of articular cartilage in the body. It means the compressive mechanical stimulation is a very important factor for formation of articular cartilage using a tissue-engineering technique. The objective of this study is to engineer cartilaginous constructs with mechano-active scaffolds and to evaluate the effect of dynamic compression for regeneration of cartilage. The mechano-active scaffolds were prepared from a very elastic poly(L-lactide-co- ε-caprolactone) (PLCL) with 85% porosity and 300-500 μm pore size using a gel-pressing method. The scaffold was seeded with 2 × 106 chondrocytes and the continuous compressive deformation of 5% strain was applied with 0.1 Hz for 10 days and 24 days, respectively. Then, the chondrocytes-seeded constructs were implanted subcutaneously into nude mice. Mechano-active scaffolds with complete rubber-like elasticity showed almost complete (over 97%) recovery at an applied strain of up to 500%. The amount of chondral extracellular matrix was increased significantly by mechanical stimulation on the highly elastic mechano-active scaffolds. Histological analysis showed the mechanically stimulated implants formed mature and well-developed cartilaginous tissue, as evidenced by the chondrocytes within lacunae and the abundant accumulation of sulfated GAGs. However, unhealthy lacunae shapes and hypertrophy forms were observed in the implants stimulated mechanically for 24 days, compared with those stimulated for 10 days. In conclusion, the proper periodical application of dynamic compression can encourage chondrocytes to maintain their phenotypes and enhance the production of GAGs, which would improve the quality of cartilaginous tissue formed both in vitro and in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 61-74 |
| Number of pages | 14 |
| Journal | Journal of Biomaterials Science, Polymer Edition |
| Volume | 19 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2008 Jan 1 |
| Externally published | Yes |
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Keywords
- Bioreactor
- Chondrocytes
- Compressive mode
- Elastic poly(L-lactide-co-ε- caprolactone) scaffold
- Mechanical stimulation
- Mechano-active tissue engineering
ASJC Scopus subject areas
- Biophysics
- Biomaterials
Cite this
Cartilaginous tissue formation using a mechano-active scaffold and dynamic compressive stimulation. / Jung, Youngmee; Kim, Soo Hyun; Kim, Sang Heon; Kim, Young Ha; Xie, Jun; Matsuda, Takehisa; Min, Byoung Goo.
In: Journal of Biomaterials Science, Polymer Edition, Vol. 19, No. 1, 01.01.2008, p. 61-74.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cartilaginous tissue formation using a mechano-active scaffold and dynamic compressive stimulation
AU - Jung, Youngmee
AU - Kim, Soo Hyun
AU - Kim, Sang Heon
AU - Kim, Young Ha
AU - Xie, Jun
AU - Matsuda, Takehisa
AU - Min, Byoung Goo
PY - 2008/1/1
Y1 - 2008/1/1
N2 - It is known that complex loading is involved in the development and maintenance of articular cartilage in the body. It means the compressive mechanical stimulation is a very important factor for formation of articular cartilage using a tissue-engineering technique. The objective of this study is to engineer cartilaginous constructs with mechano-active scaffolds and to evaluate the effect of dynamic compression for regeneration of cartilage. The mechano-active scaffolds were prepared from a very elastic poly(L-lactide-co- ε-caprolactone) (PLCL) with 85% porosity and 300-500 μm pore size using a gel-pressing method. The scaffold was seeded with 2 × 106 chondrocytes and the continuous compressive deformation of 5% strain was applied with 0.1 Hz for 10 days and 24 days, respectively. Then, the chondrocytes-seeded constructs were implanted subcutaneously into nude mice. Mechano-active scaffolds with complete rubber-like elasticity showed almost complete (over 97%) recovery at an applied strain of up to 500%. The amount of chondral extracellular matrix was increased significantly by mechanical stimulation on the highly elastic mechano-active scaffolds. Histological analysis showed the mechanically stimulated implants formed mature and well-developed cartilaginous tissue, as evidenced by the chondrocytes within lacunae and the abundant accumulation of sulfated GAGs. However, unhealthy lacunae shapes and hypertrophy forms were observed in the implants stimulated mechanically for 24 days, compared with those stimulated for 10 days. In conclusion, the proper periodical application of dynamic compression can encourage chondrocytes to maintain their phenotypes and enhance the production of GAGs, which would improve the quality of cartilaginous tissue formed both in vitro and in vivo.
AB - It is known that complex loading is involved in the development and maintenance of articular cartilage in the body. It means the compressive mechanical stimulation is a very important factor for formation of articular cartilage using a tissue-engineering technique. The objective of this study is to engineer cartilaginous constructs with mechano-active scaffolds and to evaluate the effect of dynamic compression for regeneration of cartilage. The mechano-active scaffolds were prepared from a very elastic poly(L-lactide-co- ε-caprolactone) (PLCL) with 85% porosity and 300-500 μm pore size using a gel-pressing method. The scaffold was seeded with 2 × 106 chondrocytes and the continuous compressive deformation of 5% strain was applied with 0.1 Hz for 10 days and 24 days, respectively. Then, the chondrocytes-seeded constructs were implanted subcutaneously into nude mice. Mechano-active scaffolds with complete rubber-like elasticity showed almost complete (over 97%) recovery at an applied strain of up to 500%. The amount of chondral extracellular matrix was increased significantly by mechanical stimulation on the highly elastic mechano-active scaffolds. Histological analysis showed the mechanically stimulated implants formed mature and well-developed cartilaginous tissue, as evidenced by the chondrocytes within lacunae and the abundant accumulation of sulfated GAGs. However, unhealthy lacunae shapes and hypertrophy forms were observed in the implants stimulated mechanically for 24 days, compared with those stimulated for 10 days. In conclusion, the proper periodical application of dynamic compression can encourage chondrocytes to maintain their phenotypes and enhance the production of GAGs, which would improve the quality of cartilaginous tissue formed both in vitro and in vivo.
KW - Bioreactor
KW - Chondrocytes
KW - Compressive mode
KW - Elastic poly(L-lactide-co-ε- caprolactone) scaffold
KW - Mechanical stimulation
KW - Mechano-active tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=37849001743&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37849001743&partnerID=8YFLogxK
U2 - 10.1163/156856208783227712
DO - 10.1163/156856208783227712
M3 - Article
C2 - 18177554
AN - SCOPUS:37849001743
VL - 19
SP - 61
EP - 74
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
SN - 0920-5063
IS - 1
ER -