Cartilaginous tissue formation using a mechano-active scaffold and dynamic compressive stimulation

Youngmee Jung, Soo Hyun Kim, Sang Heon Kim, Young Ha Kim, Jun Xie, Takehisa Matsuda, Byoung Goo Min

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

It is known that complex loading is involved in the development and maintenance of articular cartilage in the body. It means the compressive mechanical stimulation is a very important factor for formation of articular cartilage using a tissue-engineering technique. The objective of this study is to engineer cartilaginous constructs with mechano-active scaffolds and to evaluate the effect of dynamic compression for regeneration of cartilage. The mechano-active scaffolds were prepared from a very elastic poly(L-lactide-co- ε-caprolactone) (PLCL) with 85% porosity and 300-500 μm pore size using a gel-pressing method. The scaffold was seeded with 2 × 106 chondrocytes and the continuous compressive deformation of 5% strain was applied with 0.1 Hz for 10 days and 24 days, respectively. Then, the chondrocytes-seeded constructs were implanted subcutaneously into nude mice. Mechano-active scaffolds with complete rubber-like elasticity showed almost complete (over 97%) recovery at an applied strain of up to 500%. The amount of chondral extracellular matrix was increased significantly by mechanical stimulation on the highly elastic mechano-active scaffolds. Histological analysis showed the mechanically stimulated implants formed mature and well-developed cartilaginous tissue, as evidenced by the chondrocytes within lacunae and the abundant accumulation of sulfated GAGs. However, unhealthy lacunae shapes and hypertrophy forms were observed in the implants stimulated mechanically for 24 days, compared with those stimulated for 10 days. In conclusion, the proper periodical application of dynamic compression can encourage chondrocytes to maintain their phenotypes and enhance the production of GAGs, which would improve the quality of cartilaginous tissue formed both in vitro and in vivo.

Original languageEnglish
Pages (from-to)61-74
Number of pages14
JournalJournal of Biomaterials Science, Polymer Edition
Volume19
Issue number1
DOIs
Publication statusPublished - 2008 Jan 1
Externally publishedYes

Fingerprint

Chondrocytes
Scaffolds
Tissue
Cartilage
Articular Cartilage
Porosity
Rubber
Elasticity
Tissue Engineering
Nude Mice
Hypertrophy
Extracellular Matrix
Regeneration
Tissue engineering
Gels
Maintenance
Pore size
Phenotype
Engineers
Recovery

Keywords

  • Bioreactor
  • Chondrocytes
  • Compressive mode
  • Elastic poly(L-lactide-co-ε- caprolactone) scaffold
  • Mechanical stimulation
  • Mechano-active tissue engineering

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials

Cite this

Cartilaginous tissue formation using a mechano-active scaffold and dynamic compressive stimulation. / Jung, Youngmee; Kim, Soo Hyun; Kim, Sang Heon; Kim, Young Ha; Xie, Jun; Matsuda, Takehisa; Min, Byoung Goo.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 19, No. 1, 01.01.2008, p. 61-74.

Research output: Contribution to journalArticle

Jung, Youngmee ; Kim, Soo Hyun ; Kim, Sang Heon ; Kim, Young Ha ; Xie, Jun ; Matsuda, Takehisa ; Min, Byoung Goo. / Cartilaginous tissue formation using a mechano-active scaffold and dynamic compressive stimulation. In: Journal of Biomaterials Science, Polymer Edition. 2008 ; Vol. 19, No. 1. pp. 61-74.
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