TY - JOUR
T1 - (-)-Catechin promotes adipocyte differentiation in human bone marrow mesenchymal stem cells through PPARγ transactivation
AU - Shin, Dong Wook
AU - Kim, Su Nam
AU - Lee, Sang Min
AU - Lee, Woojung
AU - Song, Min Jeong
AU - Park, Sun Mi
AU - Lee, Tae Ryong
AU - Baik, Joo Hyun
AU - Kim, Han Kon
AU - Hong, Jeong Ho
AU - Noh, Minsoo
N1 - Funding Information:
This work was partly supported by a Korea Research Foundation Grant attributed by the Korea government (MOEHRD) (KRF-C00239), by the Research Program for New Drug Target Discovery (M10748000286-07N4800-28610), and by a grant from the Ministry of Science & Technology, South Korea.
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Green tea intake has been shown to confer various health benefits to patients suffering from metabolic disorders. Here, we studied the effect of several major green tea polyphenols on adipocyte differentiation in human bone marrow mesenchymal stem cells (hBM-MSCs) and compared it to the effect of representative antidiabetic drugs. (-)-Catechin was the most potent of the eight green tea polyphenols evaluated in promoting adipocyte differentiation in hBM-MSCs, and this effect was dose-dependent. (-)-Catechin increased the mRNA levels of various adipogenic markers, such as adiponectin, peroxisome proliferator-activated receptor gamma (PPARγ), FABP4, and LPL, as measured during adipocyte differentiation in hBM-MSCs. In addition, (-)-catechin upregulated the secretion of adiponectin in hBM-MSC culture. Using a reporter gene assay and a competitive ligand binding study, (-)-catechin also significantly activated PPARγ in a dose-dependent fashion; however, (+)-catechin, the enantiomer of (-)-catechin, was not effective as a PPARγ agonist, which seems to imply that the effect of (-)-catechin on PPARγ is stereospecific. In conclusion, our data suggest that (-)-catechin promotes adipocyte differentiation and increased sensitivity to insulin in part by direct activation of PPARγ, which could be at the basis of the observed pharmacological benefits of green tea intake in reducing the risk of type 2 diabetes.
AB - Green tea intake has been shown to confer various health benefits to patients suffering from metabolic disorders. Here, we studied the effect of several major green tea polyphenols on adipocyte differentiation in human bone marrow mesenchymal stem cells (hBM-MSCs) and compared it to the effect of representative antidiabetic drugs. (-)-Catechin was the most potent of the eight green tea polyphenols evaluated in promoting adipocyte differentiation in hBM-MSCs, and this effect was dose-dependent. (-)-Catechin increased the mRNA levels of various adipogenic markers, such as adiponectin, peroxisome proliferator-activated receptor gamma (PPARγ), FABP4, and LPL, as measured during adipocyte differentiation in hBM-MSCs. In addition, (-)-catechin upregulated the secretion of adiponectin in hBM-MSC culture. Using a reporter gene assay and a competitive ligand binding study, (-)-catechin also significantly activated PPARγ in a dose-dependent fashion; however, (+)-catechin, the enantiomer of (-)-catechin, was not effective as a PPARγ agonist, which seems to imply that the effect of (-)-catechin on PPARγ is stereospecific. In conclusion, our data suggest that (-)-catechin promotes adipocyte differentiation and increased sensitivity to insulin in part by direct activation of PPARγ, which could be at the basis of the observed pharmacological benefits of green tea intake in reducing the risk of type 2 diabetes.
KW - (-)-Catechin
KW - Adipocyte differentiation
KW - Human bone marrow mesenchymal stem cells
KW - PPARγ
KW - green tea
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U2 - 10.1016/j.bcp.2008.09.033
DO - 10.1016/j.bcp.2008.09.033
M3 - Article
C2 - 18951882
AN - SCOPUS:56649109706
VL - 77
SP - 125
EP - 133
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 1
ER -