Cathepsin B Imaging to Predict Quality of Engineered Cartilage

Ji Young Yhee, Yong Jin Kim, Ju Hee Ryu, Hong Yeol Yoon, Hyeyoun Chang, Jae Hyung Park, Hyukjin Lee, Hyon Seok Jang, Unyong Jeong, Kwang Meyung Kim, Sun Woong Kang

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Abstract

Cathepsin B (CB)-specific molecular imaging probe is applied to monitor the changes of CB expression in 3D cultured chondrocytes during the chondrogenesis. The probe is synthesized with a CB-cleavable peptide linked to a near infrared fluorescence (NIRF) dye and a dark quencher, which can recover the NIRF signal in the presence of CB enzyme. The CB activities in two different sets of chondrocytes are comparatively analyzed. The chondrocytes with higher CB activity show more extensive area of chondrogenesis that CB molecular imaging directly reflects the chondrogenic potency of the cells. The CB probe is expected to provide a reliable prediction for the quality of engineered cartilage by visualizing the activity of the relevant enzyme in chondrocytes.

Original languageEnglish
Pages (from-to)1224-1232
Number of pages9
JournalMacromolecular Bioscience
Volume15
Issue number9
DOIs
Publication statusPublished - 2015 Sep 1

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Keywords

  • cartilage tissue engineering
  • cathepsin B
  • diagnosis
  • molecular imaging
  • transplantation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Yhee, J. Y., Kim, Y. J., Ryu, J. H., Yoon, H. Y., Chang, H., Park, J. H., Lee, H., Jang, H. S., Jeong, U., Kim, K. M., & Kang, S. W. (2015). Cathepsin B Imaging to Predict Quality of Engineered Cartilage. Macromolecular Bioscience, 15(9), 1224-1232. https://doi.org/10.1002/mabi.201500215