Cathepsin B Imaging to Predict Quality of Engineered Cartilage

Ji Young Yhee, Yong Jin Kim, Ju Hee Ryu, Hong Yeol Yoon, Hyeyoun Chang, Jae Hyung Park, Hyukjin Lee, Hyon Seok Jang, Unyong Jeong, Kwang Meyung Kim, Sun Woong Kang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cathepsin B (CB)-specific molecular imaging probe is applied to monitor the changes of CB expression in 3D cultured chondrocytes during the chondrogenesis. The probe is synthesized with a CB-cleavable peptide linked to a near infrared fluorescence (NIRF) dye and a dark quencher, which can recover the NIRF signal in the presence of CB enzyme. The CB activities in two different sets of chondrocytes are comparatively analyzed. The chondrocytes with higher CB activity show more extensive area of chondrogenesis that CB molecular imaging directly reflects the chondrogenic potency of the cells. The CB probe is expected to provide a reliable prediction for the quality of engineered cartilage by visualizing the activity of the relevant enzyme in chondrocytes.

Original languageEnglish
Pages (from-to)1224-1232
Number of pages9
JournalMacromolecular Bioscience
Volume15
Issue number9
DOIs
Publication statusPublished - 2015 Sep 1

Fingerprint

Cathepsin B
Cartilage
Imaging techniques
Chondrocytes
Molecular imaging
Chondrogenesis
Molecular Imaging
Enzymes
Fluorescence
Infrared radiation
Molecular Probes
Cathepsins
Peptides
Coloring Agents
Dyes

Keywords

  • cartilage tissue engineering
  • cathepsin B
  • diagnosis
  • molecular imaging
  • transplantation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Yhee, J. Y., Kim, Y. J., Ryu, J. H., Yoon, H. Y., Chang, H., Park, J. H., ... Kang, S. W. (2015). Cathepsin B Imaging to Predict Quality of Engineered Cartilage. Macromolecular Bioscience, 15(9), 1224-1232. https://doi.org/10.1002/mabi.201500215

Cathepsin B Imaging to Predict Quality of Engineered Cartilage. / Yhee, Ji Young; Kim, Yong Jin; Ryu, Ju Hee; Yoon, Hong Yeol; Chang, Hyeyoun; Park, Jae Hyung; Lee, Hyukjin; Jang, Hyon Seok; Jeong, Unyong; Kim, Kwang Meyung; Kang, Sun Woong.

In: Macromolecular Bioscience, Vol. 15, No. 9, 01.09.2015, p. 1224-1232.

Research output: Contribution to journalArticle

Yhee, JY, Kim, YJ, Ryu, JH, Yoon, HY, Chang, H, Park, JH, Lee, H, Jang, HS, Jeong, U, Kim, KM & Kang, SW 2015, 'Cathepsin B Imaging to Predict Quality of Engineered Cartilage', Macromolecular Bioscience, vol. 15, no. 9, pp. 1224-1232. https://doi.org/10.1002/mabi.201500215
Yhee JY, Kim YJ, Ryu JH, Yoon HY, Chang H, Park JH et al. Cathepsin B Imaging to Predict Quality of Engineered Cartilage. Macromolecular Bioscience. 2015 Sep 1;15(9):1224-1232. https://doi.org/10.1002/mabi.201500215
Yhee, Ji Young ; Kim, Yong Jin ; Ryu, Ju Hee ; Yoon, Hong Yeol ; Chang, Hyeyoun ; Park, Jae Hyung ; Lee, Hyukjin ; Jang, Hyon Seok ; Jeong, Unyong ; Kim, Kwang Meyung ; Kang, Sun Woong. / Cathepsin B Imaging to Predict Quality of Engineered Cartilage. In: Macromolecular Bioscience. 2015 ; Vol. 15, No. 9. pp. 1224-1232.
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