Caulobacter profunda sp. nov., isolated from deep freshwater sediment

Long Jin, Hyun Joon La, Hyung Gwan Lee, Jay Jung Lee, Sang-Hyup Lee, Chi Yong Ahn, Hee Mock Oh

Research output: Contribution to journalArticle

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Abstract

The Gram-stain-negative, aerobic, non-spore-forming, motile, with a single polar flagellum, or non-motile (stalked) and rod-shaped bacteria, DS48-5-2T and DS48-6-3, were isolated from a sediment sample collected from a depth of 48 m taken from Daechung Reservoir, Republic of Korea. Comparative 16S rRNA gene sequence studies showed that the two isolates had clear affiliation with Alphaproteobacteria and the closest relatedness to Caulobacter mirabilis FWC 38T, Caulobacter fusiformis ATCC 15257T and Caulobacter daechungensis H-E3-2T showing 98.5 %, 97.3 % and 97.3 % 16S rRNA gene sequence similarity, respectively, and 96.1-96.7 % similarity to all other species of the genus Caulobacter. The two isolates shared 100 % 16S rRNA gene sequence similarity. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (C18: 1ω6c and/or C18: 1ω7c), C16: 0, C18: 0ω7c 11-methyl and summed feature 3 (C16: 1ω6c and/or C16: 1ω7c). The G+C contents of the genomic DNA of strains DS48-5-2T and DS48-6-3 were 66.7 mol% and 66.2 mol%, respectively. DNA-DNA hybridization values of strains DS48-5-2T and DS48-6-3 with C. mirabilis FWC 38T, C. fusiformis ATCC 15257T and C. daechungensis H-E3-2T were 19.3 %-24.4 %. Thus, based on the evidence from polyphasic studies, it is proposed that strains DS48-5-2T and DS48-6-3 are representatives of a novel species in the genus Caulobacter, for which the name Caulobacter profunda sp. nov. is proposed. The type strain is DS48-5-2T (= KCTC 32480T = JCM 19440T).

Original languageEnglish
Pages (from-to)762-767
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
Issue numberPART 3
DOIs
Publication statusPublished - 2014 Mar 17

Fingerprint

Caulobacter
freshwater sediment
Fresh Water
Sediments
Caulobacter fusiformis
DNA
sediments
gene
Genes
new species
Mirabilis
rRNA Genes
ribosomal RNA
nucleotide sequences
relatedness
Ubiquinone
genomics
fatty acid
ubiquinones
alpha-Proteobacteria

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Caulobacter profunda sp. nov., isolated from deep freshwater sediment. / Jin, Long; La, Hyun Joon; Lee, Hyung Gwan; Lee, Jay Jung; Lee, Sang-Hyup; Ahn, Chi Yong; Oh, Hee Mock.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 64, No. PART 3, 17.03.2014, p. 762-767.

Research output: Contribution to journalArticle

Jin, Long ; La, Hyun Joon ; Lee, Hyung Gwan ; Lee, Jay Jung ; Lee, Sang-Hyup ; Ahn, Chi Yong ; Oh, Hee Mock. / Caulobacter profunda sp. nov., isolated from deep freshwater sediment. In: International Journal of Systematic and Evolutionary Microbiology. 2014 ; Vol. 64, No. PART 3. pp. 762-767.
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abstract = "The Gram-stain-negative, aerobic, non-spore-forming, motile, with a single polar flagellum, or non-motile (stalked) and rod-shaped bacteria, DS48-5-2T and DS48-6-3, were isolated from a sediment sample collected from a depth of 48 m taken from Daechung Reservoir, Republic of Korea. Comparative 16S rRNA gene sequence studies showed that the two isolates had clear affiliation with Alphaproteobacteria and the closest relatedness to Caulobacter mirabilis FWC 38T, Caulobacter fusiformis ATCC 15257T and Caulobacter daechungensis H-E3-2T showing 98.5 {\%}, 97.3 {\%} and 97.3 {\%} 16S rRNA gene sequence similarity, respectively, and 96.1-96.7 {\%} similarity to all other species of the genus Caulobacter. The two isolates shared 100 {\%} 16S rRNA gene sequence similarity. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (C18: 1ω6c and/or C18: 1ω7c), C16: 0, C18: 0ω7c 11-methyl and summed feature 3 (C16: 1ω6c and/or C16: 1ω7c). The G+C contents of the genomic DNA of strains DS48-5-2T and DS48-6-3 were 66.7 mol{\%} and 66.2 mol{\%}, respectively. DNA-DNA hybridization values of strains DS48-5-2T and DS48-6-3 with C. mirabilis FWC 38T, C. fusiformis ATCC 15257T and C. daechungensis H-E3-2T were 19.3 {\%}-24.4 {\%}. Thus, based on the evidence from polyphasic studies, it is proposed that strains DS48-5-2T and DS48-6-3 are representatives of a novel species in the genus Caulobacter, for which the name Caulobacter profunda sp. nov. is proposed. The type strain is DS48-5-2T (= KCTC 32480T = JCM 19440T).",
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