Caveolin-1 is associated with VCAM-1 dependent adhesion of gastric cancer cells to endothelial cells

Jaeyoung Shin, Jongmin Kim, Byungkyu Ryu, Sung Gil Chi, Heonyong Park

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background/Aims: Cell adhesion molecules play a critical role in the invasion and metastasis of a variety of human tumors. Abnormal expression of VCAM-1 has been demonstrated to correlate with the malignant progression of gastric tumors, but the molecular mechanism underlying the VCAM-1-dependent metastasis has been rarely investigated. To explore the role for tumor cell-expressing adhesion molecules in the carcinoma-endothelium adhesion, we analyzed expression status of adhesion molecules in gastric cancer cells and its association with tumor cell capability of endothelial adhesion. Methods: Endothelial adhesion ability of gastric tumor cells was tested using calcein AM staining assay. Expression of cell surface proteins was determined by Western blot, flow cytometry, and immunofluorescence assays. RNAi-mediated knockdown of gene expression and neutralization with specific antibodies were utilized for functional analysis. Results: One of three cell lines tested was identified to be adhesive to endothelial cells and express VCAM-1. Adherence ability of the cells was dramatically decreased by neutralization of surface VCAM-1. VCAM-1 was co-localized with Caveolin-1 and siRNA-mediated knockdown of Caveolin-1 expression significantly blocked the VCAM-1-dependent cell adhesion. Conclusions: Our data imply important roles for VCAM-1 and Caveolin-1 in the regulation of metastatic potential of gastric tumor cells.

Original languageEnglish
Pages (from-to)211-220
Number of pages10
JournalCellular Physiology and Biochemistry
Volume17
Issue number5-6
DOIs
Publication statusPublished - 2006 Jan 1

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Keywords

  • Caveolin-1
  • Cell adhesion
  • Gastric cancer
  • Metastasis
  • Vascular cell adhesion molecule-1

ASJC Scopus subject areas

  • Physiology

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