Caveolin internalization by heat shock or hyperosmotic shock

Young Sun Kang, Young Gyu Ko, Jeong Sun Seo

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We investigated the cellular localization of caveolin, a landmark protein of caveolae, by indirect immunofluorescence after heat shock or hyperosmotic shock. Caveolin was internalized to the perinucleus by heat shock (43°C) and relocalized in the plasma membrane after recovery of NIH3T3 cells at 37°C for 4 h. The caveolin internalization was also observed after cells were exposed to hyperosmotic shock. Caveolin disappeared from detergent-insoluble complexes in the heat-shocked cells, but alkaline phosphatase was still there, suggesting that their responses to heat shock are quite different even though both of them were enriched in detergent- insoluble complexes of normal cells. Caveolin was internalized by the actin depolymerizer cytochalasin D, but not by the tubulin depolymerizer nocodazole. In addition, cellular exposure to hydrogen peroxide caused caveolin internalization along with disintegrated microfilaments and intact microtubules. Since cellular exposure to heat shock showed disintegrated microfilaments but intact microtubules, caveolin internalization might be due to depolymerized microfilaments. When cells were exposed to heat shock and allowed to recover for 4 h, actin depolymerization and caveolin internalization were not induced by a second heat shock, suggesting that some heat shock protein(s) might prevent actin depolymerization and caveolin internalization. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)221-228
Number of pages8
JournalExperimental Cell Research
Volume255
Issue number2
DOIs
Publication statusPublished - 2000 Mar 15

ASJC Scopus subject areas

  • Cell Biology

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