CBSX3-Trxo-2 regulates ROS generation of mitochondrial complex II (succinate dehydrogenase) in Arabidopsis

Jin Seok Shin, Won Mi So, Soo Youn Kim, Minsoo Noh, Sujin Hyoung, Kyoung Shin Yoo, Jeong Sheop Shin

Research output: Contribution to journalArticle

Abstract

Despite being toxic at a high concentrations, reactive oxygen species (ROS) play a pivotal role as signaling molecules in responses to stress and regulation of plant development. The mitochondrial electron transport chain (ETC) is the major source of ROS in cells. Although the regulation of ROS in mitochondria has been well elucidated, the protein-protein interaction-based regulation of ETC members has not been well elucidated. In this study, we identified a CBS domain-containing protein, CBSX3, and found that CBSX3 activates o-type thioredoxin (Trx-o2) in mitochondria. In addition, we found that Trx-o2 interacts with SDH1, a subunit of ETC complex II. Knockdown (KD) of CBSX3 revealed anther indehiscence due to deficient lignin deposition caused by insufficient ROS accumulation, and increased expression of genes related to cell cycle and accelerated plant growth. However, in the CBSX3-overexpression plants, ROS accumulation increased, and cell cycle-related gene expression decreased, and thereby plant growth was retarded and leaf size decreased. Moreover, KD of CBSX3 and Trx-o2 conferred resistance to mitochondria ETC inhibitors in terms of ROS release. Taken together, we suggest that CBSX3-Trx-o2 is a ROS generation regulator of mitochondria in plants and plays an important role in regulating plant development and the redox system.

Original languageEnglish
Article number110458
JournalPlant Science
Volume294
DOIs
Publication statusPublished - 2020 May

Keywords

  • CBS domain
  • Lignification
  • Mitochondria
  • ROS
  • Succinate dehydrogenase
  • Thioredoxin

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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