Cd 2+ binds to Atx1 and affects the physical interaction between Atx1 and Ccc2 in Saccharomyces cerevisiae

Dong Hyuk Heo, In Joon Baek, Hyun Jun Kang, Ji Hyun Kim, Miwha Chang, Chang Min Kang, Cheol Won Yun

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The ATX1 deletion strain of Saccharomyces cerevisiae is more resistant to Cd 2+ than the wild-type. To investigate the function of Atx1 in Cd 2+ toxicity, we used a metal-binding assay to study the interaction between Atx1 and Cd 2+ in vitro. Using circular dichroism and two-hybrid analyses, we found that Atx1 can bind Cd 2+ specifically and that Cd 2+ binding to Atx1 affects the physical interaction between Atx1 and Ccc2. These results imply that Atx1 delivers Cd 2+ to Ccc2 and that this delivery is, at least in part, responsible for Cd 2+ toxicity in S. cerevisiae.

Original languageEnglish
Pages (from-to)303-307
Number of pages5
JournalBiotechnology letters
Volume34
Issue number2
DOIs
Publication statusPublished - 2012 Feb

Keywords

  • Atx1
  • Cadmium
  • Ccc2
  • Copper
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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