CD80 (B7.1) and CD86 (B7.2) induce EBV-transformed B cell apoptosis through the Fas/FasL pathway

Ga Bin Park, Yeong Seok Kim, Hyun Kyung Lee, Dae Ho Cho, Daejin Kim, Dae Young Hur

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

CD80 and CD86 expression is strongly regulated in B cells and is induced by various stimuli (e.g., cytokines, ligation of MHC class II and CD40 ligand). Epstein-Barr virus (EBV) infection activates B lymphocytes and transforms them into lymphoblastoid cells. However, the role of CD80 and CD86 in EBV infection of B cells remains unclear. Here, we observed that cross-linking of CD80 and CD86 in EBV-transformed B cells induced apoptosis through caspase-dependent release of apoptosis-related molecules, cytochrome c and apoptosis-inducing factor (AIF) from mitochondria, because Z-VAD-fmk (N-benzyloxycarbonyl-Val-Ala- Asp-fluoromethylketone) and N-acetylcysteine (NAC) blocked apoptosis and disruption of mitochondria. Stimulation of CD80 and CD86 induced expression of Fas ligand (FasL) on EBV-transformed B cells and upregulated Fas and FasL expression in IM-9 cells. Apoptosis through Fas-FasL interactions was blocked by treatment of cells with ZB4, an antagonistic anti-Fas antibody. These results suggest that the co-stimulatory molecules CD80 and CD86 induced by EBV infection stimulate apoptosis of EBV-transformed lymphoblastoid B cells via the Fas/FasL pathway.

Original languageEnglish
Pages (from-to)1531-1540
Number of pages10
JournalInternational Journal of Oncology
Volume43
Issue number5
DOIs
Publication statusPublished - 2013 Nov 1
Externally publishedYes

Fingerprint

Fas Ligand Protein
Human Herpesvirus 4
B-Lymphocytes
Apoptosis
Epstein-Barr Virus Infections
Mitochondria
Apoptosis Inducing Factor
CD40 Ligand
Acetylcysteine
Caspases
Cytochromes c
Ligation
Anti-Idiotypic Antibodies
Cytokines

Keywords

  • Apoptosis
  • B cells
  • CD80
  • CD86
  • Epstein-Barr virus

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

CD80 (B7.1) and CD86 (B7.2) induce EBV-transformed B cell apoptosis through the Fas/FasL pathway. / Park, Ga Bin; Kim, Yeong Seok; Lee, Hyun Kyung; Cho, Dae Ho; Kim, Daejin; Hur, Dae Young.

In: International Journal of Oncology, Vol. 43, No. 5, 01.11.2013, p. 1531-1540.

Research output: Contribution to journalArticle

Park, Ga Bin ; Kim, Yeong Seok ; Lee, Hyun Kyung ; Cho, Dae Ho ; Kim, Daejin ; Hur, Dae Young. / CD80 (B7.1) and CD86 (B7.2) induce EBV-transformed B cell apoptosis through the Fas/FasL pathway. In: International Journal of Oncology. 2013 ; Vol. 43, No. 5. pp. 1531-1540.
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