CD8+ T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax

Hyo Jin Kim, Bong Kwang Jung, Jin Joo Lee, Kyoung Ho Pyo, Tae Yun Kim, Byung il Choi, Tae Woo Kim, Hajime Hisaeda, Kunisuke Himeno, Eun Hee Shin, Jong Yil Chai

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2 Citations (Scopus)

Abstract

Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8+ T-cells and CD4+ T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8+ cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique.

Original languageEnglish
Pages (from-to)85-90
Number of pages6
JournalKorean Journal of Parasitology
Volume49
Issue number1
DOIs
Publication statusPublished - 2011 Mar

Keywords

  • Apical membrane antigen (AMA)
  • CD8 T-cell
  • DNA vaccine
  • Gene gun
  • Plasmodium vivax

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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    Kim, H. J., Jung, B. K., Lee, J. J., Pyo, K. H., Kim, T. Y., Choi, B. I., Kim, T. W., Hisaeda, H., Himeno, K., Shin, E. H., & Chai, J. Y. (2011). CD8+ T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax. Korean Journal of Parasitology, 49(1), 85-90. https://doi.org/10.3347/kjp.2011.49.1.85