CDK4 down-regulation induced by paclitaxel is associated with G1 arrest in gastric cancer cells

Young Do Yoo, Jong Kuk Park, Ju Youn Choi, Kee Ho Lee, Yoon Koo Kang, Chong Suk Kim, Sang Won Shin, Yeul Hong Kim, Jun Suk Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Paclitaxel induces a cell cycle block at G2-M phase by preventing the depolymerization of microtubules and induces p53-independent apoptosis in many cancer cells. We observed that gastric cancer cells treated with paclitaxel have shown a cyclin-dependent kinase (CDK)4 down-regulation. This paclitaxel-induced CDK4 down-regulation resulted in a cell cycle arrest at G1-S phase. To confirm this observation, we prepared stable transfectants that overexpressed CDK4 and analyzed the cell cycle progression. Ectopic expression of CDK4 in SNU cells resulted in a release of paclitaxel-induced G1 arrest. The release of G1 arrest by enforced expression of CDK4 seems to make the cells more sensitive to paclitaxel-induced apoptosis. From this finding, we could then suggest that paclitaxel treatment induces both G1-S and G2-M blocks in the cell cycle progression of gastric cancer cells.

Original languageEnglish
Pages (from-to)3063-3068
Number of pages6
JournalClinical Cancer Research
Volume4
Issue number12
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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