cDNA-AFLP analysis of 1BL.1RS under water-deficit stress and development of wheat-rye translocation-specific markers

Ji Hee Jang, Woo Joo Jung, Dae Yeon Kim, Yong Weon Seo

Research output: Contribution to journalArticle

Abstract

Drought is a major stressor that severely hampers the production of many important crops. The short arm of rye (Secale cereale) chromosome 1 contains favourable stress-resistance alleles and is thus widely used in wheat (Triticum aestivum) breeding to generate 1BL.1RS translocation lines. In this study, 1BL.1RS-specific drought-responsive genes were identified using cDNA-amplified fragment length polymorphism (AFLP) analysis. Among the 144 transcript-derived fragments (TDF) differentially expressed in the 1BL.1RS line, we identified the function and chromosomal position of 84 TDF using public databases. Real-time PCR was performed to validate the results of cDNA-AFLP, and four TDF were significantly up-regulated. Furthermore, we developed two wheat-rye translocation-specific markers by comparing the TDF from the short arms of wheat and rye chromosome 1. One is specific to 1BL.1RS and the other is specific to 1AL.1RS and 1BL.1RS. Combinational use of both markers will be helpful to breed wheat possessing 1RS.

Original languageEnglish
Pages (from-to)1-15
Number of pages15
JournalNew Zealand Journal of Crop and Horticultural Science
DOIs
Publication statusAccepted/In press - 2016 Dec 20

Fingerprint

rye
amplified fragment length polymorphism
wheat
water
drought
translocation lines
chromosomes
Secale cereale
stress tolerance
quantitative polymerase chain reaction
Triticum aestivum
alleles
breeds
breeding
crops
genes

Keywords

  • 1BL.1RS
  • cDNA-AFLP
  • drought tolerance
  • molecular marker
  • Triticum aestivum

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture

Cite this

cDNA-AFLP analysis of 1BL.1RS under water-deficit stress and development of wheat-rye translocation-specific markers. / Jang, Ji Hee; Jung, Woo Joo; Kim, Dae Yeon; Seo, Yong Weon.

In: New Zealand Journal of Crop and Horticultural Science, 20.12.2016, p. 1-15.

Research output: Contribution to journalArticle

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