cDNA microarray analysis of the differential gene expression in the neuropathic pain and electroacupuncture treatment models

Je Sang Ko, Sun Na Doe, Han Lee Young, Young Shin Soon, Hoon Kim Ji, Gil Hwang Byung, Byung Il Min, Suk Park Dong

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Partial nerve injury is the main cause of neuropathic pain disorders in humans. Acupuncture has long been used to relieve pain. It is known to relieve pain by controlling the activities of the autonomic nervous system. Although the mechanism of neuropathic pain and analgesic effects of electroacupuncture (EA) have been studied in a rat model system, its detailed mechanism at the molecular level remains unclear. To identify genes that might serve as either markers or explain these distinct biological functions, a cDNA microarray analysis was used to compare the expression of 8,400 genes among three sample groups. Messenger RNAs that were pooled from the spinal nerves of 7 normal, 7 neuropathic pain, and 7 EA treatment rat models were compared. Sixty-eight genes were differentially expressed more than 2-fold in the neuropathic rat model when compared to the normal, and restored to the normal expression level after the EA treatment. These genes are involved in a number of biological processes, including the signal transduction, gene expression, and nociceptive pathways. Confirmation of the differential gene expression was performed by a dotblot analysis. Dot-blotting results showed that the opioid receptor sigma was among those genes. This indicates that opioid-signaling events are involved in neuropathic pain and the analgesic effects of EA. The potential application of these data include the identification and characterization of signaling pathways that are involved in the EA treatment, studies on the role of the opioid receptor in neuropathic pain, and further exploration on the role of selected identified genes in animal models.

Original languageEnglish
Pages (from-to)420-427
Number of pages8
JournalJournal of Biochemistry and Molecular Biology
Volume35
Issue number4
Publication statusPublished - 2002 Jul 1
Externally publishedYes

Fingerprint

Electroacupuncture
Neuralgia
Microarray Analysis
Microarrays
Oligonucleotide Array Sequence Analysis
Gene expression
Complementary DNA
Genes
Gene Expression
Rats
Analgesics
Therapeutics
Acupuncture
sigma Receptors
Biological Phenomena
Pain
Spinal Nerves
Signal transduction
Somatoform Disorders
Autonomic Nervous System

Keywords

  • Electroacupuncture
  • Microarray
  • Neuropathic pain
  • Opioid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

cDNA microarray analysis of the differential gene expression in the neuropathic pain and electroacupuncture treatment models. / Ko, Je Sang; Doe, Sun Na; Young, Han Lee; Soon, Young Shin; Ji, Hoon Kim; Byung, Gil Hwang; Min, Byung Il; Dong, Suk Park.

In: Journal of Biochemistry and Molecular Biology, Vol. 35, No. 4, 01.07.2002, p. 420-427.

Research output: Contribution to journalArticle

Ko, Je Sang ; Doe, Sun Na ; Young, Han Lee ; Soon, Young Shin ; Ji, Hoon Kim ; Byung, Gil Hwang ; Min, Byung Il ; Dong, Suk Park. / cDNA microarray analysis of the differential gene expression in the neuropathic pain and electroacupuncture treatment models. In: Journal of Biochemistry and Molecular Biology. 2002 ; Vol. 35, No. 4. pp. 420-427.
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