Cell age optimization for hydrogen production induced by sulfur deprivation using a green alga Chlamydomonas reinhardtii UTEX 90

Jun Pyo Kim, Chang Duk Kang, Sang Jun Sim, Mi Sun Kim, Tai Hyun Park, Donghyun Lee, Dukjoon Kim, Ji Heung Kim, Young Kwan Lee, Daewon Pak

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26 Citations (Scopus)

Abstract

Under sulfur deprived conditions, PS II and photosynthetic O2 evolution by Chlamydomonas reinhardtii UTEX 90 are inactivated, resulting in shift from aerobic to anaerobic condition. This is followed by hydrogen production catalyzed by hydrogenase. We hypothesized that the photosynthetic capacity and the accumulation of endogenous substrates such as starch for hydrogen production might be different according to cell age. Accordingly, we investigated (a) the relationships between hydrogen production, induction time of sulfur deprivation, increase of chlorophyll after sulfur deprivation, and residual PS II activity, and (b) the effect of initial cell density upon sulfur deprivation. The maximum production volume of hydrogen was 151 ml H2/l with 0.91 g/l of cell density in the late-exponential phase. We suggest that the effects of induction time and initial cell density at sulfur deprivation on hydrogen production, up to an optimal concentration, are due to an increase of chlorophyll under sulfur deprivation.

Original languageEnglish
Pages (from-to)131-135
Number of pages5
JournalJournal of microbiology and biotechnology
Volume15
Issue number1
Publication statusPublished - 2005 Feb
Externally publishedYes

Keywords

  • Cell growth stage
  • Chlamydomonas reinhardtii
  • Hydrogen production
  • Sulfur deprivation

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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    Kim, J. P., Kang, C. D., Sim, S. J., Kim, M. S., Park, T. H., Lee, D., Kim, D., Kim, J. H., Lee, Y. K., & Pak, D. (2005). Cell age optimization for hydrogen production induced by sulfur deprivation using a green alga Chlamydomonas reinhardtii UTEX 90. Journal of microbiology and biotechnology, 15(1), 131-135.