Abstract
Aim: Lactic acid bacteria (LAB) are beneficial micro-organisms that have been associated with several probiotic effects in both humans and animals. Here, using proteome analysis, we investigate the antitumour effects of cell-bound exopolysaccharides (cb-EPS) isolated from Lactobacillus acidophilus 606 on colon cancer cells and explore the proteins critical for their antitumour activity. Methods and Results: cb-EPS inhibited the proliferation of HT-29 colon cancer cells by directly affecting cell morphology and not the cell cycle. Using two-dimensional polyacrylamide gel electrophoresis coupled with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and immunoblot analysis, we found that cb-EPS dramatically induced Beclin-1 and GRP78, and affected Bcl-2 and Bak regulation. Conclusions: The results of this study indicate that cb-EPS are antitumourigenic against HT-29 colon cancer cells and that this activity is because of the activation of autophagic cell death promoted directly by the induction of Beclin-1 and GRP78, as well as indirectly through the induction of Bcl-2 and Bak. Significance and Impact of the Study: These results may contribute to understanding the novel mechanisms by which probiotic bacteria induce tumour cell death via autophagy.
| Original language | English |
|---|---|
| Pages (from-to) | 123-130 |
| Number of pages | 8 |
| Journal | Letters in Applied Microbiology |
| Volume | 51 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2010 Aug 1 |
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Keywords
- antitumour activity
- autophagy
- cb-EPS
- two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
Cite this
Cell-bound exopolysaccharide from probiotic bacteria induces autophagic cell death of tumour cells. / Kim, Y.; Oh, S.; Yun, H. S.; Oh, S.; Kim, Sae Hun.
In: Letters in Applied Microbiology, Vol. 51, No. 2, 01.08.2010, p. 123-130.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell-bound exopolysaccharide from probiotic bacteria induces autophagic cell death of tumour cells
AU - Kim, Y.
AU - Oh, S.
AU - Yun, H. S.
AU - Oh, S.
AU - Kim, Sae Hun
PY - 2010/8/1
Y1 - 2010/8/1
N2 - Aim: Lactic acid bacteria (LAB) are beneficial micro-organisms that have been associated with several probiotic effects in both humans and animals. Here, using proteome analysis, we investigate the antitumour effects of cell-bound exopolysaccharides (cb-EPS) isolated from Lactobacillus acidophilus 606 on colon cancer cells and explore the proteins critical for their antitumour activity. Methods and Results: cb-EPS inhibited the proliferation of HT-29 colon cancer cells by directly affecting cell morphology and not the cell cycle. Using two-dimensional polyacrylamide gel electrophoresis coupled with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and immunoblot analysis, we found that cb-EPS dramatically induced Beclin-1 and GRP78, and affected Bcl-2 and Bak regulation. Conclusions: The results of this study indicate that cb-EPS are antitumourigenic against HT-29 colon cancer cells and that this activity is because of the activation of autophagic cell death promoted directly by the induction of Beclin-1 and GRP78, as well as indirectly through the induction of Bcl-2 and Bak. Significance and Impact of the Study: These results may contribute to understanding the novel mechanisms by which probiotic bacteria induce tumour cell death via autophagy.
AB - Aim: Lactic acid bacteria (LAB) are beneficial micro-organisms that have been associated with several probiotic effects in both humans and animals. Here, using proteome analysis, we investigate the antitumour effects of cell-bound exopolysaccharides (cb-EPS) isolated from Lactobacillus acidophilus 606 on colon cancer cells and explore the proteins critical for their antitumour activity. Methods and Results: cb-EPS inhibited the proliferation of HT-29 colon cancer cells by directly affecting cell morphology and not the cell cycle. Using two-dimensional polyacrylamide gel electrophoresis coupled with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and immunoblot analysis, we found that cb-EPS dramatically induced Beclin-1 and GRP78, and affected Bcl-2 and Bak regulation. Conclusions: The results of this study indicate that cb-EPS are antitumourigenic against HT-29 colon cancer cells and that this activity is because of the activation of autophagic cell death promoted directly by the induction of Beclin-1 and GRP78, as well as indirectly through the induction of Bcl-2 and Bak. Significance and Impact of the Study: These results may contribute to understanding the novel mechanisms by which probiotic bacteria induce tumour cell death via autophagy.
KW - antitumour activity
KW - autophagy
KW - cb-EPS
KW - two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)
UR - http://www.scopus.com/inward/record.url?scp=77954474565&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954474565&partnerID=8YFLogxK
U2 - 10.1111/j.1472-765X.2010.02859.x
DO - 10.1111/j.1472-765X.2010.02859.x
M3 - Article
C2 - 20536712
AN - SCOPUS:77954474565
VL - 51
SP - 123
EP - 130
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
SN - 0266-8254
IS - 2
ER -