Cell-cell adhesion and RhoA-mediated actin polymerization are independent phenomena in microtubule disrupted keratinocytes

Sun-Ho Kee, Shyh Ing Jang, Bijan Ahvazi, Melinda Larsen, Kenneth M. Yamada, Peter M. Steinert

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

E-cadherin-mediated adherens junction formation and maintenance are thought to involve actin filament rearrangements through the action of small GTPases. Recently, we demonstrated that microtubule disruption in normal human epidermal keratinocytes grown in low calcium media conditions induces cell-cell adhesion by redistribution of endogenous E-cadherin, and it promotes stress fiber formation. This actin rearrangement was apparently mediated by RhoA activation. This model system therefore provides a tool with which to dissect relationships between cell-cell adhesion and Rho-mediated stress fiber formation. In this study, we have demonstrated in normal human epidermal keratinocytes that disruption of actin structures including stress fibers does not interfere with E-cadherin redistribution during microtubule-induced cell-cell adhesion. Moreover, this cell-cell adhesion could not be blocked by RhoA inactivation at the level for inhibition of stress fiber formation. Additionally, in the immortalized HaCaT keratinocyte cell line, which does not undergo cell-cell adhesion after microtubule disruption in low calcium conditions, expression of dominant-active RhoA could induce stress fiber formation without inducing adhesion. On the other hand, a variant of the HaCaT cell line, HC-R1, showed microtubule-disruption-induced cell-cell adhesion without stress fiber formation. Together, our results suggest that, in keratinocytes, the process of cell adhesion can occur independently of RhoA-mediated stress fiber formation.

Original languageEnglish
Pages (from-to)440-448
Number of pages9
JournalJournal of Investigative Dermatology
Volume119
Issue number2
DOIs
Publication statusPublished - 2002 Sep 20
Externally publishedYes

Fingerprint

Stress Fibers
Cell adhesion
Keratinocytes
Cell Adhesion
Microtubules
Polymerization
Actins
Fibers
Cadherins
Cells
Calcium
Adherens Junctions
Cell Line
Monomeric GTP-Binding Proteins
Actin Cytoskeleton
Adhesion
Chemical activation
Maintenance

Keywords

  • Actin
  • Adherens junction
  • E-cadherin

ASJC Scopus subject areas

  • Dermatology

Cite this

Cell-cell adhesion and RhoA-mediated actin polymerization are independent phenomena in microtubule disrupted keratinocytes. / Kee, Sun-Ho; Jang, Shyh Ing; Ahvazi, Bijan; Larsen, Melinda; Yamada, Kenneth M.; Steinert, Peter M.

In: Journal of Investigative Dermatology, Vol. 119, No. 2, 20.09.2002, p. 440-448.

Research output: Contribution to journalArticle

Kee, Sun-Ho ; Jang, Shyh Ing ; Ahvazi, Bijan ; Larsen, Melinda ; Yamada, Kenneth M. ; Steinert, Peter M. / Cell-cell adhesion and RhoA-mediated actin polymerization are independent phenomena in microtubule disrupted keratinocytes. In: Journal of Investigative Dermatology. 2002 ; Vol. 119, No. 2. pp. 440-448.
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