Cell cycle-dependent regulation of Aurora kinase B mRNA by the Microprocessor complex

Eunsun Jung; Youngmo Seong; Jae Hong Seo; Young Soo Kwon; Hoseok Song

doi:10.1016/j.bbrc.2014.02.104

Cell cycle-dependent regulation of Aurora kinase B mRNA by the Microprocessor complex

Eunsun Jung, Youngmo Seong, Jae Hong Seo, Young Soo Kwon, Hoseok Song

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Aurora kinase B regulates the segregation of chromosomes and the spindle checkpoint during mitosis. In this study, we showed that the Microprocessor complex, which is responsible for the processing of the primary transcripts during the generation of microRNAs, destabilizes the mRNA of Aurora kinase B in human cells. The Microprocessor-mediated cleavage kept Aurora kinase B at a low level and prevented premature entrance into mitosis. The cleavage was reduced during mitosis leading to the accumulation of Aurora kinase B mRNA and protein. In addition to Aurora kinase B mRNA, the processing of other primary transcripts of miRNAs were also decreased during mitosis. We found that the cleavage was dependent on an RNA helicase, DDX5, and the association of DDX5 and DDX17 with the Microprocessor was reduced during mitosis. Thus, we propose a novel mechanism by which the Microprocessor complex regulates stability of Aurora kinase B mRNA and cell cycle progression.

Original language	English
Pages (from-to)	241-247
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	446
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2014.02.104
Publication status	Published - 2014 Mar 28

Keywords

Aurora kinase B
Cell cycle
Mitosis
The Microprocessor complex

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2014.02.104

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title = "Cell cycle-dependent regulation of Aurora kinase B mRNA by the Microprocessor complex",

abstract = "Aurora kinase B regulates the segregation of chromosomes and the spindle checkpoint during mitosis. In this study, we showed that the Microprocessor complex, which is responsible for the processing of the primary transcripts during the generation of microRNAs, destabilizes the mRNA of Aurora kinase B in human cells. The Microprocessor-mediated cleavage kept Aurora kinase B at a low level and prevented premature entrance into mitosis. The cleavage was reduced during mitosis leading to the accumulation of Aurora kinase B mRNA and protein. In addition to Aurora kinase B mRNA, the processing of other primary transcripts of miRNAs were also decreased during mitosis. We found that the cleavage was dependent on an RNA helicase, DDX5, and the association of DDX5 and DDX17 with the Microprocessor was reduced during mitosis. Thus, we propose a novel mechanism by which the Microprocessor complex regulates stability of Aurora kinase B mRNA and cell cycle progression.",

keywords = "Aurora kinase B, Cell cycle, Mitosis, The Microprocessor complex",

author = "Eunsun Jung and Youngmo Seong and Seo, {Jae Hong} and Kwon, {Young Soo} and Hoseok Song",

note = "Funding Information: This work was supported by a Grant of the National Research Foundation of Korea (2012R1A1A2003267) & a Korea University Grant to H.S. and a Grant of the National Research Foundation of Korea (NRF-2009-0068994) to Y.K. ",

year = "2014",

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doi = "10.1016/j.bbrc.2014.02.104",

language = "English",

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journal = "Biochemical and Biophysical Research Communications",

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AU - Jung, Eunsun

AU - Seong, Youngmo

AU - Seo, Jae Hong

AU - Kwon, Young Soo

AU - Song, Hoseok

N1 - Funding Information: This work was supported by a Grant of the National Research Foundation of Korea (2012R1A1A2003267) & a Korea University Grant to H.S. and a Grant of the National Research Foundation of Korea (NRF-2009-0068994) to Y.K.

PY - 2014/3/28

Y1 - 2014/3/28

N2 - Aurora kinase B regulates the segregation of chromosomes and the spindle checkpoint during mitosis. In this study, we showed that the Microprocessor complex, which is responsible for the processing of the primary transcripts during the generation of microRNAs, destabilizes the mRNA of Aurora kinase B in human cells. The Microprocessor-mediated cleavage kept Aurora kinase B at a low level and prevented premature entrance into mitosis. The cleavage was reduced during mitosis leading to the accumulation of Aurora kinase B mRNA and protein. In addition to Aurora kinase B mRNA, the processing of other primary transcripts of miRNAs were also decreased during mitosis. We found that the cleavage was dependent on an RNA helicase, DDX5, and the association of DDX5 and DDX17 with the Microprocessor was reduced during mitosis. Thus, we propose a novel mechanism by which the Microprocessor complex regulates stability of Aurora kinase B mRNA and cell cycle progression.

AB - Aurora kinase B regulates the segregation of chromosomes and the spindle checkpoint during mitosis. In this study, we showed that the Microprocessor complex, which is responsible for the processing of the primary transcripts during the generation of microRNAs, destabilizes the mRNA of Aurora kinase B in human cells. The Microprocessor-mediated cleavage kept Aurora kinase B at a low level and prevented premature entrance into mitosis. The cleavage was reduced during mitosis leading to the accumulation of Aurora kinase B mRNA and protein. In addition to Aurora kinase B mRNA, the processing of other primary transcripts of miRNAs were also decreased during mitosis. We found that the cleavage was dependent on an RNA helicase, DDX5, and the association of DDX5 and DDX17 with the Microprocessor was reduced during mitosis. Thus, we propose a novel mechanism by which the Microprocessor complex regulates stability of Aurora kinase B mRNA and cell cycle progression.

KW - Aurora kinase B

KW - Cell cycle

KW - Mitosis

KW - The Microprocessor complex

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JO - Biochemical and Biophysical Research Communications

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Cell cycle-dependent regulation of Aurora kinase B mRNA by the Microprocessor complex

Abstract

Keywords

ASJC Scopus subject areas

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