Cell cycle-dependent regulation of Aurora kinase B mRNA by the Microprocessor complex

Eunsun Jung, Youngmo Seong, Jae Hong Seo, Young Soo Kwon, Hoseok Song

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aurora kinase B regulates the segregation of chromosomes and the spindle checkpoint during mitosis. In this study, we showed that the Microprocessor complex, which is responsible for the processing of the primary transcripts during the generation of microRNAs, destabilizes the mRNA of Aurora kinase B in human cells. The Microprocessor-mediated cleavage kept Aurora kinase B at a low level and prevented premature entrance into mitosis. The cleavage was reduced during mitosis leading to the accumulation of Aurora kinase B mRNA and protein. In addition to Aurora kinase B mRNA, the processing of other primary transcripts of miRNAs were also decreased during mitosis. We found that the cleavage was dependent on an RNA helicase, DDX5, and the association of DDX5 and DDX17 with the Microprocessor was reduced during mitosis. Thus, we propose a novel mechanism by which the Microprocessor complex regulates stability of Aurora kinase B mRNA and cell cycle progression.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume446
Issue number1
DOIs
Publication statusPublished - 2014 Mar 28

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Keywords

  • Aurora kinase B
  • Cell cycle
  • Mitosis
  • The Microprocessor complex

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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