Cell Cycle Dysregulation Induced by Cytoplasm of Lactococcus lactis ssp. lactis in SNUC2A, a Colon Cancer Cell Line

Ji Yeon Kim; Hee Jong Woo; Young Suk Kim; Kyoung Heon Kim; Hyong Joo Lee

doi:10.1207/S15327914NC4602_13

Cell Cycle Dysregulation Induced by Cytoplasm of Lactococcus lactis ssp. lactis in SNUC2A, a Colon Cancer Cell Line

Ji Yeon Kim, Hee Jong Woo, Young Suk Kim, Kyoung Heon Kim, Hyong Joo Lee

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

The anticancer effect of cytoplasmic fraction from Lactococcus lactis ssp. lactis, which had showed strong antiproliferative activity against SNUC2A human colon cancer cell line in the previous study, was investigated. The proliferation of SNUC2A was inhibited by the treatment with cytoplasmic fraction of Lactococcus lactis ssp. lactis in a dose-dependent and partially reversible manner. After exposure to the cytoplasmic fraction of Lc. lactis for 72 h, strong antiproliferative activity was efficiently induced through S-phase accumulation in SNUC2A cells. Analysis of cell cycle regulatory proteins demonstrated that the cytoplasmic fraction enhanced the levels of p21 ^CIP1 and cyclin A, decreased cyclin E protein, and slightly reduced the activity of cyclin-dependent kinase 2 (CDK2).

Original language	English
Pages (from-to)	197-201
Number of pages	5
Journal	Nutrition and Cancer
Volume	46
Issue number	2
DOIs	https://doi.org/10.1207/S15327914NC4602_13
Publication status	Published - 2003

ASJC Scopus subject areas

Medicine (miscellaneous)
Oncology
Nutrition and Dietetics
Cancer Research

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10.1207/S15327914NC4602_13

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title = "Cell Cycle Dysregulation Induced by Cytoplasm of Lactococcus lactis ssp. lactis in SNUC2A, a Colon Cancer Cell Line",

abstract = "The anticancer effect of cytoplasmic fraction from Lactococcus lactis ssp. lactis, which had showed strong antiproliferative activity against SNUC2A human colon cancer cell line in the previous study, was investigated. The proliferation of SNUC2A was inhibited by the treatment with cytoplasmic fraction of Lactococcus lactis ssp. lactis in a dose-dependent and partially reversible manner. After exposure to the cytoplasmic fraction of Lc. lactis for 72 h, strong antiproliferative activity was efficiently induced through S-phase accumulation in SNUC2A cells. Analysis of cell cycle regulatory proteins demonstrated that the cytoplasmic fraction enhanced the levels of p21 CIP1 and cyclin A, decreased cyclin E protein, and slightly reduced the activity of cyclin-dependent kinase 2 (CDK2).",

author = "Kim, {Ji Yeon} and Woo, {Hee Jong} and Kim, {Young Suk} and Kim, {Kyoung Heon} and Lee, {Hyong Joo}",

note = "Funding Information: This study was supported by a grant of the Korea Health 21 R&D Project (HMP-98-F-5-0016) from Ministry of Health and Welfare, Republic of Korea. Address correspondence to H. J. Lee, Department of Food Science and Technology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151–742, South Korea. Phone: 82-2-880-4853. FAX: 82-2-873-5095. E-mail: leehyjo@snu.ac.kr.",

year = "2003",

doi = "10.1207/S15327914NC4602_13",

language = "English",

volume = "46",

pages = "197--201",

journal = "Nutrition and Cancer",

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number = "2",

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TY - JOUR

T1 - Cell Cycle Dysregulation Induced by Cytoplasm of Lactococcus lactis ssp. lactis in SNUC2A, a Colon Cancer Cell Line

AU - Kim, Ji Yeon

AU - Woo, Hee Jong

AU - Kim, Young Suk

AU - Kim, Kyoung Heon

AU - Lee, Hyong Joo

N1 - Funding Information: This study was supported by a grant of the Korea Health 21 R&D Project (HMP-98-F-5-0016) from Ministry of Health and Welfare, Republic of Korea. Address correspondence to H. J. Lee, Department of Food Science and Technology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151–742, South Korea. Phone: 82-2-880-4853. FAX: 82-2-873-5095. E-mail: leehyjo@snu.ac.kr.

PY - 2003

Y1 - 2003

N2 - The anticancer effect of cytoplasmic fraction from Lactococcus lactis ssp. lactis, which had showed strong antiproliferative activity against SNUC2A human colon cancer cell line in the previous study, was investigated. The proliferation of SNUC2A was inhibited by the treatment with cytoplasmic fraction of Lactococcus lactis ssp. lactis in a dose-dependent and partially reversible manner. After exposure to the cytoplasmic fraction of Lc. lactis for 72 h, strong antiproliferative activity was efficiently induced through S-phase accumulation in SNUC2A cells. Analysis of cell cycle regulatory proteins demonstrated that the cytoplasmic fraction enhanced the levels of p21 CIP1 and cyclin A, decreased cyclin E protein, and slightly reduced the activity of cyclin-dependent kinase 2 (CDK2).

AB - The anticancer effect of cytoplasmic fraction from Lactococcus lactis ssp. lactis, which had showed strong antiproliferative activity against SNUC2A human colon cancer cell line in the previous study, was investigated. The proliferation of SNUC2A was inhibited by the treatment with cytoplasmic fraction of Lactococcus lactis ssp. lactis in a dose-dependent and partially reversible manner. After exposure to the cytoplasmic fraction of Lc. lactis for 72 h, strong antiproliferative activity was efficiently induced through S-phase accumulation in SNUC2A cells. Analysis of cell cycle regulatory proteins demonstrated that the cytoplasmic fraction enhanced the levels of p21 CIP1 and cyclin A, decreased cyclin E protein, and slightly reduced the activity of cyclin-dependent kinase 2 (CDK2).

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U2 - 10.1207/S15327914NC4602_13

DO - 10.1207/S15327914NC4602_13

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AN - SCOPUS:0345059356

VL - 46

SP - 197

EP - 201

JO - Nutrition and Cancer

JF - Nutrition and Cancer

SN - 0163-5581

IS - 2

ER -

Cell Cycle Dysregulation Induced by Cytoplasm of Lactococcus lactis ssp. lactis in SNUC2A, a Colon Cancer Cell Line

Abstract

ASJC Scopus subject areas

UN SDGs

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