TY - JOUR
T1 - Cell Cycle Dysregulation Induced by Cytoplasm of Lactococcus lactis ssp. lactis in SNUC2A, a Colon Cancer Cell Line
AU - Kim, Ji Yeon
AU - Woo, Hee Jong
AU - Kim, Young Suk
AU - Kim, Kyoung Heon
AU - Lee, Hyong Joo
N1 - Funding Information:
This study was supported by a grant of the Korea Health 21 R&D Project (HMP-98-F-5-0016) from Ministry of Health and Welfare, Republic of Korea. Address correspondence to H. J. Lee, Department of Food Science and Technology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151–742, South Korea. Phone: 82-2-880-4853. FAX: 82-2-873-5095. E-mail: leehyjo@snu.ac.kr.
PY - 2003
Y1 - 2003
N2 - The anticancer effect of cytoplasmic fraction from Lactococcus lactis ssp. lactis, which had showed strong antiproliferative activity against SNUC2A human colon cancer cell line in the previous study, was investigated. The proliferation of SNUC2A was inhibited by the treatment with cytoplasmic fraction of Lactococcus lactis ssp. lactis in a dose-dependent and partially reversible manner. After exposure to the cytoplasmic fraction of Lc. lactis for 72 h, strong antiproliferative activity was efficiently induced through S-phase accumulation in SNUC2A cells. Analysis of cell cycle regulatory proteins demonstrated that the cytoplasmic fraction enhanced the levels of p21 CIP1 and cyclin A, decreased cyclin E protein, and slightly reduced the activity of cyclin-dependent kinase 2 (CDK2).
AB - The anticancer effect of cytoplasmic fraction from Lactococcus lactis ssp. lactis, which had showed strong antiproliferative activity against SNUC2A human colon cancer cell line in the previous study, was investigated. The proliferation of SNUC2A was inhibited by the treatment with cytoplasmic fraction of Lactococcus lactis ssp. lactis in a dose-dependent and partially reversible manner. After exposure to the cytoplasmic fraction of Lc. lactis for 72 h, strong antiproliferative activity was efficiently induced through S-phase accumulation in SNUC2A cells. Analysis of cell cycle regulatory proteins demonstrated that the cytoplasmic fraction enhanced the levels of p21 CIP1 and cyclin A, decreased cyclin E protein, and slightly reduced the activity of cyclin-dependent kinase 2 (CDK2).
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U2 - 10.1207/S15327914NC4602_13
DO - 10.1207/S15327914NC4602_13
M3 - Article
C2 - 14690796
AN - SCOPUS:0345059356
VL - 46
SP - 197
EP - 201
JO - Nutrition and Cancer
JF - Nutrition and Cancer
SN - 0163-5581
IS - 2
ER -
