Cell damage during femtosecond optical trapping

Kang Bin Im, Sumin Han, Hwajoon Park, Dan Jin, Soo Kie Kim, Beop Min Kim

Research output: Contribution to journalConference article

Abstract

Microparticles including biological cells were trapped and manipulated using a continuous wave tweezer and a femtosecond laser tweezer. The difference of the optical trapping force between CW and femtosecond optical tweezers increased as the particle size decreased, possibly due to the self-focusing effect of the ultrashort pulses. Also, the white damage spots were generated near the focus during femtosecond optical trapping of biological cells even with extremely low average power. The instantaneous optical damage threshold was measured as a function of the trap depth as well. These results may help to optimize the optical trapping of biological cells using femtosecond lasers.

Original languageEnglish
Article number40
Pages (from-to)274-280
Number of pages7
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5699
DOIs
Publication statusPublished - 2005 Jul 21
EventImaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III - San Jose, CA, United States
Duration: 2005 Jan 242005 Jan 27

Keywords

  • Femtosecond lasers
  • Laser damage
  • Optical trapping
  • Tweezers

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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