Cell elongation is regulated through a central circuit of interacting transcription factors in the Arabidopsis hypocotyl

Eunkyoo Oh, Jia Ying Zhu, Ming Yi Bai, Rafael Augusto Arenhart, Yu Sun, Zhi Yong Wang

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

As the major mechanism of plant growth and morphogenesis, cell elongation is controlled by many hormonal and environmental signals. How these signals are coordinated at the molecular level to ensure coherent cellular responses remains unclear. Here, we illustrate a molecular circuit that integrates all major growth-regulating signals, including auxin, brassinosteroid, gibberellin, light, and temperature. Analyses of genome-wide targets, genetic and biochemical interactions demonstrate that the auxin-response factor ARF6, the light/temperature-regulated transcription factor PIF4, and the brassinosteroid-signaling transcription factor BZR1, interact with each other and cooperatively regulate large numbers of common target genes, but their DNA-binding activities are blocked by the gibberellin-inactivated repressor RGA. In addition, tripartite HLH/bHLH module feedback-regulates PIFs and additional bHLH factors that interact with ARF6, and thereby modulates auxin sensitivity according to developmental and environmental cues. Our results demonstrate a central growth-regulation circuit that integrates hormonal, environmental, and developmental controls of cell elongation in Arabidopsis hypocotyl.

Original languageEnglish
Article numbere03031
JournaleLife
Volume2014
Issue number3
DOIs
Publication statusPublished - 2014 Mar 27
Externally publishedYes

Fingerprint

Hypocotyl
Indoleacetic Acids
Brassinosteroids
Arabidopsis
Gibberellins
Elongation
Transcription Factors
Networks (circuits)
Growth
Genes
Light
Temperature
Plant Development
Cues
Molecular Biology
Genome
Feedback
DNA

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Cell elongation is regulated through a central circuit of interacting transcription factors in the Arabidopsis hypocotyl. / Oh, Eunkyoo; Zhu, Jia Ying; Bai, Ming Yi; Arenhart, Rafael Augusto; Sun, Yu; Wang, Zhi Yong.

In: eLife, Vol. 2014, No. 3, e03031, 27.03.2014.

Research output: Contribution to journalArticle

Oh, Eunkyoo ; Zhu, Jia Ying ; Bai, Ming Yi ; Arenhart, Rafael Augusto ; Sun, Yu ; Wang, Zhi Yong. / Cell elongation is regulated through a central circuit of interacting transcription factors in the Arabidopsis hypocotyl. In: eLife. 2014 ; Vol. 2014, No. 3.
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