Cell growth and oxygen transfer in Methylosinus trichosporium OB3b cultures

Jeewon Lee, Bhupendra K. Soni, Robert L. Kelley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The steady-state concentration of M. trichosporium OB3b increased about two-fold in the continuous culture when the feeding medium was supplemented by ferrous sulphate (50 mg/L) and citric acid (100 mg/L) at a steady state. In batch and continuous cultures, the cell growth was significantly inhibited by excess N-sources (NH40H, NH4CI, NH4N03, HN03, and NaN03) and ammonium N-sources were more inhibitory. Both volumetric O2 transfer coefficient and specific 02 uptake rate increased monotonously in an extensive range of air flow rate (0.1-7 vvm) and the methane interfered with the 02 transfer even at very low flow rates (0.01-0.1 vvm).

Original languageEnglish
Pages (from-to)903-908
Number of pages6
JournalBiotechnology Letters
Volume18
Issue number8
Publication statusPublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

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