Cell growth and oxygen transfer in Methylosinus trichosporium OB3b cultures

Jeewon Lee, Bhupendra K. Soni, Robert L. Kelley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The steady-state concentration of M. trichosporium OB3b increased about two-fold in the continuous culture when the feeding medium was supplemented by ferrous sulphate (50 mg/L) and citric acid (100 mg/L) at a steady state. In batch and continuous cultures, the cell growth was significantly inhibited by excess N-sources (NH40H, NH4CI, NH4N03, HN03, and NaN03) and ammonium N-sources were more inhibitory. Both volumetric O2 transfer coefficient and specific 02 uptake rate increased monotonously in an extensive range of air flow rate (0.1-7 vvm) and the methane interfered with the 02 transfer even at very low flow rates (0.01-0.1 vvm).

Original languageEnglish
Pages (from-to)903-908
Number of pages6
JournalBiotechnology Letters
Volume18
Issue number8
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

ferrous sulfate
Methylosinus trichosporium
Batch Cell Culture Techniques
Methane
Cell growth
Ammonium Compounds
Citric Acid
Air
Flow rate
Oxygen
Citric acid
Growth
Cell culture

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Cell growth and oxygen transfer in Methylosinus trichosporium OB3b cultures. / Lee, Jeewon; Soni, Bhupendra K.; Kelley, Robert L.

In: Biotechnology Letters, Vol. 18, No. 8, 1996, p. 903-908.

Research output: Contribution to journalArticle

Lee, Jeewon ; Soni, Bhupendra K. ; Kelley, Robert L. / Cell growth and oxygen transfer in Methylosinus trichosporium OB3b cultures. In: Biotechnology Letters. 1996 ; Vol. 18, No. 8. pp. 903-908.
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