Cell-line dependency in cerebral organoid induction: cautionary observations in Alzheimer’s disease patient-derived induced pluripotent stem cells

Ju Hyun Lee, Geon Yoo, Juhyun Choi, Si Hyung Park, Hyogeun Shin, Renuka Prasad, Yeunehee Lee, Mee Ryung Ahn, Il Joo Cho, Woong Sun

Research output: Contribution to journalArticlepeer-review

Abstract

The cerebral organoid (CO) model has been used in the study of various neurodegenerative diseases owing to its physiological implications. However, the CO model may only be representative of certain clinical findings in affected patients, while some features are not recapitulated. In this study, we found that neurons in the CO model from patients with Alzheimer’s disease were less responsive to depolarization, in contrast to previous reports. This difference may be partly attributed to the variations in brain spatial identity depending on the genetic background of the induced pluripotent stem cells. Our current observation raises concerns that the phenotypes observed in the CO model need to be carefully evaluated for their clinical implications.

Original languageEnglish
Article number46
JournalMolecular brain
Volume15
Issue number1
DOIs
Publication statusPublished - 2022 Dec

Keywords

  • Alzheimer’s diseases
  • Cell-to-cell variation
  • Cerebral organoid
  • Disease modeling

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Fingerprint

Dive into the research topics of 'Cell-line dependency in cerebral organoid induction: cautionary observations in Alzheimer’s disease patient-derived induced pluripotent stem cells'. Together they form a unique fingerprint.

Cite this