Cell-specific and temporal aspects of gene expression in the chicken oviduct at different stages of the laying cycle

Wooyoung Jeong, Whasun Lim, Jinyoung Kim, Suzie E. Ahn, Hyung Chul Lee, Jae Wook Jeong, Jae Yong Han, Gwonhwa Song, Fuller W. Bazer

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Egg formation and embryonic development occur as the yolk passes through the magnum, isthmus, and shell gland of the oviduct before oviposition in hens. The present study identified candidate genes associated with secretory function of the chicken oviduct after ovulation and contributing to egg formation and oviposition. Hens (n = 5 per time point) were euthanized to recover the reproductive tract when the egg was in the magnum (3 h after ovulation) and the shell gland (20 h after ovulation). Total RNA was extracted from each segment of the oviducts and subjected to Affymetrix chicken GeneChip analysis. Quantitative PCR and in situ hybridization analyses of selected genes confirmed the validity of the gene expression patterns detected using microarray analysis. In particular, ACP1, CALB1, CYP26A1, PENK, RCAN1 and SPP1 expression increased significantly in the shell gland between 3 h and 20 h postovulation, whereas only RCNA1 expression increased significantly in the magnum between 3 h and 20 h postovulation. Results of the high-throughput analysis revealed cell-specific and temporal changes in gene expression in the oviduct at 3 h and 20 h postovulation in laying hens provide novel insight into changes at the molecular and cellular levels of candidate genes related to formation of the egg and oviposition.

Original languageEnglish
Article number172
JournalBiology of Reproduction
Volume86
Issue number6
DOIs
Publication statusPublished - 2012 Jun 1
Externally publishedYes

Fingerprint

Oviducts
Oviposition
Ovum
Chickens
Ovulation
Gene Expression
Genes
Microarray Analysis
Embryonic Development
In Situ Hybridization
RNA
Polymerase Chain Reaction

Keywords

  • Chicken
  • Egg
  • Microarray analysis
  • Oviduct
  • Ovulation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cell-specific and temporal aspects of gene expression in the chicken oviduct at different stages of the laying cycle. / Jeong, Wooyoung; Lim, Whasun; Kim, Jinyoung; Ahn, Suzie E.; Lee, Hyung Chul; Jeong, Jae Wook; Han, Jae Yong; Song, Gwonhwa; Bazer, Fuller W.

In: Biology of Reproduction, Vol. 86, No. 6, 172, 01.06.2012.

Research output: Contribution to journalArticle

Jeong, Wooyoung ; Lim, Whasun ; Kim, Jinyoung ; Ahn, Suzie E. ; Lee, Hyung Chul ; Jeong, Jae Wook ; Han, Jae Yong ; Song, Gwonhwa ; Bazer, Fuller W. / Cell-specific and temporal aspects of gene expression in the chicken oviduct at different stages of the laying cycle. In: Biology of Reproduction. 2012 ; Vol. 86, No. 6.
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