Cell-specific expression and signal transduction of C-C motif chemokine ligand 2 and atypical chemokine receptors in the porcine endometrium during early pregnancy

Whasun Lim, Hyocheol Bae, Fuller W. Bazer, Gwonhwa Song

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Chemokines and atypical chemokine receptors (ACKRs; also known as chemokine decoy receptors) play an important role in reproductive immunology by recruiting leukocytes during early pregnancy. The aim of this study was to determine the expression of C-C motif chemokine ligand 2 (CCL2) and ACKRs in the endometrium during estrous cycle and early pregnancy, and to investigate the functional effects of CCL2 on porcine uterine luminal epithelial (pLE) cells. Our results indicated that CCL2, ACKR1, ACKR3, and ACKR4 were strongly detected in the glandular and luminal epithelium of the endometrium during early pregnancy compared to that in non-pregnant pigs. Recombinant CCL2 improved pLE cell proliferation via activation of the PI3K and MAPK pathways and suppression of endoplasmic reticulum (ER) stress by reducing the expression of ER stress regulatory genes. Collectively, these results provide novel insights into CCL2-mediated signaling mechanisms in the porcine endometrium at the maternal-fetal interface during early pregnancy.

Original languageEnglish
Pages (from-to)312-323
Number of pages12
JournalDevelopmental and Comparative Immunology
Volume81
DOIs
Publication statusPublished - 2018 Apr 1

Fingerprint

Chemokine CCL2
Chemokine Receptors
Endometrium
Signal Transduction
Swine
Pregnancy
Endoplasmic Reticulum Stress
Epithelial Cells
Estrous Cycle
Regulator Genes
Allergy and Immunology
Phosphatidylinositol 3-Kinases
Chemokines
Leukocytes
Epithelium
Mothers
Cell Proliferation

Keywords

  • ACKR
  • CCL2
  • Endometrium
  • Luminal epithelial cell
  • Porcine
  • Proliferation

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

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title = "Cell-specific expression and signal transduction of C-C motif chemokine ligand 2 and atypical chemokine receptors in the porcine endometrium during early pregnancy",
abstract = "Chemokines and atypical chemokine receptors (ACKRs; also known as chemokine decoy receptors) play an important role in reproductive immunology by recruiting leukocytes during early pregnancy. The aim of this study was to determine the expression of C-C motif chemokine ligand 2 (CCL2) and ACKRs in the endometrium during estrous cycle and early pregnancy, and to investigate the functional effects of CCL2 on porcine uterine luminal epithelial (pLE) cells. Our results indicated that CCL2, ACKR1, ACKR3, and ACKR4 were strongly detected in the glandular and luminal epithelium of the endometrium during early pregnancy compared to that in non-pregnant pigs. Recombinant CCL2 improved pLE cell proliferation via activation of the PI3K and MAPK pathways and suppression of endoplasmic reticulum (ER) stress by reducing the expression of ER stress regulatory genes. Collectively, these results provide novel insights into CCL2-mediated signaling mechanisms in the porcine endometrium at the maternal-fetal interface during early pregnancy.",
keywords = "ACKR, CCL2, Endometrium, Luminal epithelial cell, Porcine, Proliferation",
author = "Whasun Lim and Hyocheol Bae and Bazer, {Fuller W.} and Gwonhwa Song",
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T1 - Cell-specific expression and signal transduction of C-C motif chemokine ligand 2 and atypical chemokine receptors in the porcine endometrium during early pregnancy

AU - Lim, Whasun

AU - Bae, Hyocheol

AU - Bazer, Fuller W.

AU - Song, Gwonhwa

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Chemokines and atypical chemokine receptors (ACKRs; also known as chemokine decoy receptors) play an important role in reproductive immunology by recruiting leukocytes during early pregnancy. The aim of this study was to determine the expression of C-C motif chemokine ligand 2 (CCL2) and ACKRs in the endometrium during estrous cycle and early pregnancy, and to investigate the functional effects of CCL2 on porcine uterine luminal epithelial (pLE) cells. Our results indicated that CCL2, ACKR1, ACKR3, and ACKR4 were strongly detected in the glandular and luminal epithelium of the endometrium during early pregnancy compared to that in non-pregnant pigs. Recombinant CCL2 improved pLE cell proliferation via activation of the PI3K and MAPK pathways and suppression of endoplasmic reticulum (ER) stress by reducing the expression of ER stress regulatory genes. Collectively, these results provide novel insights into CCL2-mediated signaling mechanisms in the porcine endometrium at the maternal-fetal interface during early pregnancy.

AB - Chemokines and atypical chemokine receptors (ACKRs; also known as chemokine decoy receptors) play an important role in reproductive immunology by recruiting leukocytes during early pregnancy. The aim of this study was to determine the expression of C-C motif chemokine ligand 2 (CCL2) and ACKRs in the endometrium during estrous cycle and early pregnancy, and to investigate the functional effects of CCL2 on porcine uterine luminal epithelial (pLE) cells. Our results indicated that CCL2, ACKR1, ACKR3, and ACKR4 were strongly detected in the glandular and luminal epithelium of the endometrium during early pregnancy compared to that in non-pregnant pigs. Recombinant CCL2 improved pLE cell proliferation via activation of the PI3K and MAPK pathways and suppression of endoplasmic reticulum (ER) stress by reducing the expression of ER stress regulatory genes. Collectively, these results provide novel insights into CCL2-mediated signaling mechanisms in the porcine endometrium at the maternal-fetal interface during early pregnancy.

KW - ACKR

KW - CCL2

KW - Endometrium

KW - Luminal epithelial cell

KW - Porcine

KW - Proliferation

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