Cellular behavior in micropatterned hydrogels by bioprinting system depended on the cell types and cellular interaction

Soyoung Hong, Seung Joon Song, Jae Yeon Lee, Hwanseok Jang, Jaesoon Choi, Kyung Sun, Yongdoo Park

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The fabrication of patterned microstructures within three-dimensional (3D) matrices is a challenging subject in tissue engineering and regenerative medicine. A 3D, free-moving bioprinting system was developed and hydrogels were patterned by varying the process parameters of z-axis moving velocity and ejection velocity. The patterning of hydrogel based microfibers in a 3D matrigel was achieved with dimensions of 4.5mm length and widths from 79 to 200μm. Hyaluronan-based hydrogels mixed with fibroblasts (L929), mouse endothelial cells (MS1), or human mesenchymal stem cells (hMSCs) were patterned using a 3D moving axis bioprinter and cell behavior was monitored in culture for up to 16 days. L929 and MS1 cells and hMSCs in patterned hydrogel revealed cell-cell interactions and a morphological dependency on cell types. HMSCs formed spheres through cell aggregation, while L929 cells increased in cellular mass without cell aggregation and MS1 dispersed into the matrix instead of aggregating. The aggregation of hMSCs was attenuated by treatment with Rho kinase (ROCK) inhibitor and cadherin antibody. This reflected the close relationship between cell aggregation and migration with RhoA and cell-cell adhesion molecules. Angiogenic-specific gene expression profiles showed that expression of CD105 decreased to 22% in the ROCK inhibitor group compared to control group. These results showed that cell-based patterns in a 3D matrix are highly dependent on both cell aggregation and migration over time.

Original languageEnglish
Pages (from-to)224-230
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume116
Issue number2
DOIs
Publication statusPublished - 2013 Aug 1

Fingerprint

Bioprinting
Hydrogels
Cell Communication
Agglomeration
Cell Aggregation
Stem cells
Hydrogel
Mesenchymal Stromal Cells
Cell Movement
rho-Associated Kinases
Cell adhesion
Endothelial cells
Cell Adhesion Molecules
Hyaluronic Acid
Cadherins
Fibroblasts
Tissue engineering
Gene expression
Antibodies
Regenerative Medicine

Keywords

  • Bioprinting
  • Cell aggregation
  • Cell migration
  • Cell-cell interaction
  • Hydrogel patterning

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Cellular behavior in micropatterned hydrogels by bioprinting system depended on the cell types and cellular interaction. / Hong, Soyoung; Song, Seung Joon; Lee, Jae Yeon; Jang, Hwanseok; Choi, Jaesoon; Sun, Kyung; Park, Yongdoo.

In: Journal of Bioscience and Bioengineering, Vol. 116, No. 2, 01.08.2013, p. 224-230.

Research output: Contribution to journalArticle

Hong, Soyoung ; Song, Seung Joon ; Lee, Jae Yeon ; Jang, Hwanseok ; Choi, Jaesoon ; Sun, Kyung ; Park, Yongdoo. / Cellular behavior in micropatterned hydrogels by bioprinting system depended on the cell types and cellular interaction. In: Journal of Bioscience and Bioengineering. 2013 ; Vol. 116, No. 2. pp. 224-230.
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