Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant

Okhil Kumar Nag, Han Young Woo, Wei R. Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N- bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.

Original languageEnglish
Title of host publicationBiophotonics and Immune Responses VII
DOIs
Publication statusPublished - 2012 Apr 2
Externally publishedYes
EventBiophotonics and Immune Responses VII - San Francisco, CA, United States
Duration: 2012 Jan 232012 Jan 23

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8224
ISSN (Print)1605-7422

Conference

ConferenceBiophotonics and Immune Responses VII
CountryUnited States
CitySan Francisco, CA
Period12/1/2312/1/23

Fingerprint

Cationic surfactants
Photons
Surface-Active Agents
Block copolymers
copolymers
surfactants
membranes
Membranes
probes
photons
Molecular Probes
Nanostructures
Cell membranes
activity (biology)
Benzene
Quantum efficiency
Encapsulation
Cell Communication
Cytosol
quantum efficiency

Keywords

  • Cellular internalization
  • Nanocarrier
  • Polymeric vesicles
  • Two-photon microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Nag, O. K., Woo, H. Y., & Chen, W. R. (2012). Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant. In Biophotonics and Immune Responses VII [82240G] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8224). https://doi.org/10.1117/12.901836

Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant. / Nag, Okhil Kumar; Woo, Han Young; Chen, Wei R.

Biophotonics and Immune Responses VII. 2012. 82240G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8224).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nag, OK, Woo, HY & Chen, WR 2012, Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant. in Biophotonics and Immune Responses VII., 82240G, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8224, Biophotonics and Immune Responses VII, San Francisco, CA, United States, 12/1/23. https://doi.org/10.1117/12.901836
Nag OK, Woo HY, Chen WR. Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant. In Biophotonics and Immune Responses VII. 2012. 82240G. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.901836
Nag, Okhil Kumar ; Woo, Han Young ; Chen, Wei R. / Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant. Biophotonics and Immune Responses VII. 2012. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
@inproceedings{16825a7129c145a2be3094cdbea8f1d9,
title = "Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant",
abstract = "We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N- bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.",
keywords = "Cellular internalization, Nanocarrier, Polymeric vesicles, Two-photon microscopy",
author = "Nag, {Okhil Kumar} and Woo, {Han Young} and Chen, {Wei R.}",
year = "2012",
month = "4",
day = "2",
doi = "10.1117/12.901836",
language = "English",
isbn = "9780819488671",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Biophotonics and Immune Responses VII",

}

TY - GEN

T1 - Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant

AU - Nag, Okhil Kumar

AU - Woo, Han Young

AU - Chen, Wei R.

PY - 2012/4/2

Y1 - 2012/4/2

N2 - We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N- bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.

AB - We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N- bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.

KW - Cellular internalization

KW - Nanocarrier

KW - Polymeric vesicles

KW - Two-photon microscopy

UR - http://www.scopus.com/inward/record.url?scp=84859090211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859090211&partnerID=8YFLogxK

U2 - 10.1117/12.901836

DO - 10.1117/12.901836

M3 - Conference contribution

SN - 9780819488671

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Biophotonics and Immune Responses VII

ER -