Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant

Okhil Kumar Nag, Han Young Woo, Wei R. Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N- bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.

Original languageEnglish
Title of host publicationBiophotonics and Immune Responses VII
DOIs
Publication statusPublished - 2012
Externally publishedYes
EventBiophotonics and Immune Responses VII - San Francisco, CA, United States
Duration: 2012 Jan 232012 Jan 23

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8224
ISSN (Print)1605-7422

Conference

ConferenceBiophotonics and Immune Responses VII
CountryUnited States
CitySan Francisco, CA
Period12/1/2312/1/23

Keywords

  • Cellular internalization
  • Nanocarrier
  • Polymeric vesicles
  • Two-photon microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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