Cellular iron utilization is regulated by putative siderophore transporter FgSit1 not by free iron transporter in Fusarium graminearum

Yong Sung Park, Tae Hyoung Kim, Hyo Ihl Chang, Ha Chin Sung, Cheol Won Yun

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This report investigated FgSit1, which encodes a putative ferrichrome transporter of Fusarium graminearum. The identity of the deduced amino acid sequence of FgSit1 with the amino acid sequence of ScArn1p, an FC-Fe3+ transporter of Saccharomyces cerevisiae, was 51%; both the growth defect related to the Δfet3Δarn1-4 strain of S. cerevisiae in an iron-depleted condition and the FC-Fe3+ uptake activity were recovered upon the introduction of FgSit1 into the Δfet3Δarn1-4 strain. Although ScArn1p was found in the late endosomal compartment in S. cerevisiae, FgSit1 was found on the plasma membrane in S. cerevisiae; when FgSit1 was expressed exogenously in S. cerevisiae, it showed greater FC-Fe3+ uptake activity than did ScArn1p. Additionally, in F. graminearum FC-Fe3+ uptake activity in the Δfgsit1 strain was found to be one-fourth that of the wild-type. However, Fe3+ uptake activity in the Δfgsit1 strain was 5-fold higher than that of wild-type; the gene expression of FgFtr1, a putative iron transporter, was induced by the deletion of FgSit1, but was not induced by the deletion of FgSit2. Taken together, these results strongly suggest that FgSit1 encodes a putative FC-Fe3+ transporter that mediates FC-Fe3+ uptake using a different mechanism than ScArn1p and plays an important role in the regulation of cellular iron availability in F. graminearum.

Original languageEnglish
Pages (from-to)1634-1642
Number of pages9
JournalBiochemical and biophysical research communications
Volume345
Issue number4
DOIs
Publication statusPublished - 2006 Jul 14

Keywords

  • F. graminearum
  • Iron
  • S. cerevisiae
  • Siderophore
  • Transporter

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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