Cellular organization of three germ layer cells on different types of noncovalent functionalized graphene substrates

Yong Ju Yun, Soon Jung Park, Joseph Seo, Yun Ho Song, Dong Han Ha, Hyung Min Chung, Yongseok Jun, Sung Hwan Moon

Research output: Contribution to journalArticle

Abstract

Graphene and its derivatives have seen a rapid rise in interest as promising biomaterials especially in the field of tissue engineering, regenerative medicine, and cell biology of late. Despite its proven potential in numerous biological applications, information regarding the relationship between the different forms of graphene and cell lineages is still lacking partly due to its topical emergence in cellular studies. Herein, we explore the biocompatibility of four types of graphene substrates (chemical vapor deposition grown graphene, mechanically exfoliated graphene, chemically exfoliated graphene oxide, and reduced graphene oxide) with three types of somatic cells (keratinocytes, hepatocytes, endothelial cells) derived from the three germ layers in relation to cell adhesion, proliferation, morphology, and gene expression. The results revealed exceptional cell adhesion for all tested groups but enhanced proliferation and cytoskeletal interconnectivity in graphene oxide and reduced graphene oxide substrates. We were unable to detect any adverse effects in gene expression and survivability during a week of culture. We further show topographic changes to graphene substrates under fetal bovine serum adsorption to better illustrate the actual microenvironment of inhabitant cells. This study highlights the extraordinary synergy between graphene and somatic cells, suggesting the discretionary use of extracellular matrix components for in vitro cultivation.

Original languageEnglish
Article number109729
JournalMaterials Science and Engineering C
Volume103
DOIs
Publication statusPublished - 2019 Oct 1

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Graphite
Graphene
graphene
Substrates
cells
Oxides
oxides
gene expression
Cell adhesion
Gene expression
adhesion
Cytology
inhabitants
tissue engineering
Endothelial cells
Biocompatible Materials
biocompatibility
Biocompatibility
medicine
Tissue engineering

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Cellular organization of three germ layer cells on different types of noncovalent functionalized graphene substrates. / Yun, Yong Ju; Park, Soon Jung; Seo, Joseph; Song, Yun Ho; Ha, Dong Han; Chung, Hyung Min; Jun, Yongseok; Moon, Sung Hwan.

In: Materials Science and Engineering C, Vol. 103, 109729, 01.10.2019.

Research output: Contribution to journalArticle

Yun, Yong Ju ; Park, Soon Jung ; Seo, Joseph ; Song, Yun Ho ; Ha, Dong Han ; Chung, Hyung Min ; Jun, Yongseok ; Moon, Sung Hwan. / Cellular organization of three germ layer cells on different types of noncovalent functionalized graphene substrates. In: Materials Science and Engineering C. 2019 ; Vol. 103.
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