Cellular resistance to adriamycin conferred by enhanced Rb expression is associated with increased MDR1 expression

Young Do Yoo, Seongman Kang, Yoon Koo Kang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In order to investigate if the enhanced expression of Rb confers increased cellular resistance to adriamycin, we made Rb stable transfectants from colon carcinoma cells, SW620. Rb stable transfectants exhibited 5- to 10-fold more resistance to adriamycin than the control cells. To study the correlation between enhanced Rb expression and MDR1 expression, products of the Rb gene and the MDR1 gene in Rb stable transfectants were measured by Western blot analysis. These Rb transfectants showed increased MDR1 expression. Transient transfection of the MDR1 promoter-CAT reporter gene and the Rb gene demonstrated that Rb up-regulated MDR1 promoter activity in SW620 cells. Rb may, at least partly, contribute to a role in protecting cells from carcinogen exposure by up-regulating the MDR1 gene.

Original languageEnglish
Pages (from-to)6-10
Number of pages5
JournalBiochemical and biophysical research communications
Volume249
Issue number1
DOIs
Publication statusPublished - 1998 Aug 10

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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