Cellular responses to nanotopology of polymeric surfaces fabricated with AAO nanoimprinting

Donghwan Kim, S. Y. Park, Yongdoo Park, J. H. Kim, Kyung Sun, G. H. Lee, Ki Bong Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

We made various patterns on the PMMA surfaces and observed the cellular responses on them. Nanoimprinting using AAO (anodized aluminum oxide) template was used for the topology control of polymeric culture surfaces. AAO has numerous regularly-spaced nanopores. The pore diameter and the center-to-center pore distance can be easily controlled by altering the anodizing and etching conditions. One of the main advantages of using AAO as a template in nanoimprinting is that nanometerscale structure templates larger than 25 cm2 are easily, economically and quickly fabricated, which are suitable for various cellular studies. We cultured fibroblast on the variously nanopatterned surfaces directly or after surface treatment with collagen, O2 plasma, or both. The cellular activities of fibroblasts on 420nm-nodule pattern treated with both were significantly retarded at 24 hours. Fibroblasts also showed significantly different activities among different patterns. In the time-lapse observation of initial adhesion in stressed condition (ambient temperature and no CO2 supply) from 3.5 hours to 5.5 hours after seeding, we could conclude that fibroblast do not like 420nm-nodule pattern for their initial adhesion as fibroblasts on 420nm-nodule pattern markedly changed their shape to round form while those on 90nm-nodule pattern maintained their shape.

Original languageEnglish
Title of host publication2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Pages188-189
Number of pages2
Volume2005
DOIs
Publication statusPublished - 2005 Dec 1
Event2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology - Oahu, HI, United States
Duration: 2005 May 122005 May 15

Other

Other2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
CountryUnited States
CityOahu, HI
Period05/5/1205/5/15

    Fingerprint

Keywords

  • AAO
  • Cellular resoponse
  • Nanoimprinting
  • Nanopattern
  • Nanotopology

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kim, D., Park, S. Y., Park, Y., Kim, J. H., Sun, K., Lee, G. H., & Lee, K. B. (2005). Cellular responses to nanotopology of polymeric surfaces fabricated with AAO nanoimprinting. In 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology (Vol. 2005, pp. 188-189). [1548422] https://doi.org/10.1109/MMB.2005.1548422