Cellulolytic Enzymes Production by Utilizing Agricultural Wastes Under Solid State Fermentation and its Application for Biohydrogen Production

Ganesh D. Saratale, Siddheshwar D. Kshirsagar, Vilas T. Sampange, Rijuta G. Saratale, Sang Eun Oh, Sanjay P. Govindwar, Min-Kyu Oh

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Phanerochaete chrysosporium was evaluated for cellulase and hemicellulase production using various agricultural wastes under solid state fermentation. Optimization of various environmental factors, type of substrate, and medium composition was systematically investigated to maximize the production of enzyme complex. Using grass powder as a carbon substrate, maximum activities of endoglucanase (188.66 U/gds), exoglucanase (24.22 U/gds), cellobiase (244.60 U/gds), filter paperase (FPU) (30.22 U/gds), glucoamylase (505.0 U/gds), and xylanase (427.0 U/gds) were produced under optimized conditions. The produced crude enzyme complex was employed for hydrolysis of untreated and mild acid pretreated rice husk. The maximum amount of reducing sugar released from enzyme treated rice husk was 485 mg/g of the substrate. Finally, the hydrolysates of rice husk were used for hydrogen production by Clostridium beijerinckii. The maximum cumulative H2 production and H2 yield were 237.97 mL and 2.93 mmoL H2/g of reducing sugar, (or 2.63 mmoL H2/g of cellulose), respectively. Biohydrogen production performance obtained from this work is better than most of the reported results from relevant studies. The present study revealed the cost-effective process combining cellulolytic enzymes production under solid state fermentation (SSF) and the conversion of agro-industrial residues into renewable energy resources.

Original languageEnglish
Pages (from-to)2801-2817
Number of pages17
JournalApplied Biochemistry and Biotechnology
Volume174
Issue number8
DOIs
Publication statusPublished - 2014 Jan 1

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Solid Waste
Agricultural wastes
Fermentation
Enzymes
Cellulase
Clostridium beijerinckii
Sugars
Renewable Energy
Phanerochaete
Glucan 1,4-alpha-Glucosidase
Substrates
beta-Glucosidase
Renewable energy resources
Poaceae
Clostridium
Cellulose
Powders
Hydrogen
Hydrogen production
Hydrolysis

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Cellulolytic Enzymes Production by Utilizing Agricultural Wastes Under Solid State Fermentation and its Application for Biohydrogen Production. / Saratale, Ganesh D.; Kshirsagar, Siddheshwar D.; Sampange, Vilas T.; Saratale, Rijuta G.; Oh, Sang Eun; Govindwar, Sanjay P.; Oh, Min-Kyu.

In: Applied Biochemistry and Biotechnology, Vol. 174, No. 8, 01.01.2014, p. 2801-2817.

Research output: Contribution to journalArticle

Saratale, Ganesh D. ; Kshirsagar, Siddheshwar D. ; Sampange, Vilas T. ; Saratale, Rijuta G. ; Oh, Sang Eun ; Govindwar, Sanjay P. ; Oh, Min-Kyu. / Cellulolytic Enzymes Production by Utilizing Agricultural Wastes Under Solid State Fermentation and its Application for Biohydrogen Production. In: Applied Biochemistry and Biotechnology. 2014 ; Vol. 174, No. 8. pp. 2801-2817.
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